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RETRACTED ARTICLE: Virus-induced gene silencing for functional analysis of selected genes

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An Erratum to this article was published on 16 December 2008

Abstract

Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism and has been shown to be of great potential in plant reverse genetics. Circumvention of plant transformation, methodological simplicity, robustness, and speedy results makes VIGS an attractive alternative instrument in functional genomics, even in a high throughput fashion. The system is well established in Nicotiana benthamiana, and efforts are being made to improve VIGS in other species, including monocots. Here, we discuss the issues specific to the application of VIGS technology to determine gene function, which has revealed the roles of a variety of genes in disease resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis.

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Authors and Affiliations

  1. Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, 117543, Singapore

    Mandar R. Godge & Prakash P. Kumar

  2. Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India

    Arunima Purkayastha & Indranil Dasgupta

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  1. Mandar R. Godge
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  2. Arunima Purkayastha
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  3. Indranil Dasgupta
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  4. Prakash P. Kumar
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Correspondence to Indranil Dasgupta or Prakash P. Kumar.

Additional information

Communicated by P. Puigdomenech.

M. R. Godge and A. Purkayastha made equal contributions and hence should be treated as joint first authors for this paper.

This article was retracted due to copyright violation.

An erratum to this article is available at http://dx.doi.org/10.1007/s00299-008-0660-4.

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Godge, M.R., Purkayastha, A., Dasgupta, I. et al. RETRACTED ARTICLE: Virus-induced gene silencing for functional analysis of selected genes. Plant Cell Rep 27, 209–219 (2008). https://doi.org/10.1007/s00299-007-0460-2

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