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Identification and characterization of COI1-dependent transcription factor genes involved in JA-mediated response to wounding in Arabidopsis plants

  • Physiology and Biochemistry
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Abstract

The phytohormone jasmonic acid (JA) is an important signaling molecular involved in many developmental and physiological processes, especially in the response of plants to wounding. In this study, we adopted a new strategy, taking into consideration the microarray data of the CHX treatment, to identify 15 COI1-dependent JA-inducible transcription factors (JCTFs) that have distinct expression patterns in response to wounding. After the analysis on the JCTFs over-expressor plants, we identified four JCTFs, i.e., WRKY18, At1g74930 and At3g53600 in addition to AtMYC2, as the positive regulators in the JA-mediated signaling pathway in response to Arabidopsis wounding.

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Abbreviations

JCTF:

JA and CHX induced transcription factor

JA:

Jasmonic acid

MeJA:

Methyl jasmonic acid

ABA:

Abscisic acid

SA:

Salicylic acid

GA:

Gibberellin

NPR1:

Non-expressor of PR1

OPDA:

12-Oxophytodienoic acid

CHX:

Cyclohexmide

coi1 :

coronatine insensitive1

PAL:

Phenylalanine ammonia-lyase

VSP2:

Vegetative storage protein 2

LOX3:

Lipoxygenase 3

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Acknowledgments

The authors thank Prof. Dao-Xin Xie (Tsinghua University, China) and Dr. Haruko Okamoto (Oxford University, UK) for critical comments and valuable discussion. This work was supported by a NSFC grant (GN 30625002).

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Authors and Affiliations

  1. National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing, 100871, China

    Zhe Wang, Guangyu Cao, Xinlei Wang, Jin Miao, Xiaoting Liu, Zhangliang Chen, Li-Jia Qu & Hongya Gu

  2. The National Plant Gene Research Center (Beijing), Beijing, 100101, China

    Zhangliang Chen, Li-Jia Qu & Hongya Gu

Authors
  1. Zhe Wang
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  2. Guangyu Cao
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  3. Xinlei Wang
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  4. Jin Miao
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  5. Xiaoting Liu
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  6. Zhangliang Chen
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  7. Li-Jia Qu
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  8. Hongya Gu
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Corresponding author

Correspondence to Hongya Gu.

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Communicated by W.-H. Wu.

Zhe Wang and Guangyu Cao have contributed equally.

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Wang, Z., Cao, G., Wang, X. et al. Identification and characterization of COI1-dependent transcription factor genes involved in JA-mediated response to wounding in Arabidopsis plants. Plant Cell Rep 27, 125–135 (2008). https://doi.org/10.1007/s00299-007-0410-z

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  • DOI: https://doi.org/10.1007/s00299-007-0410-z

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