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High responsiveness in de novo shoot organogenesis induction of Passiflora cristalina (Passifloraceae), a wild Amazonian passion fruit species

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Abstract

The aim of the present study was to establish a regeneration system via de novo organogenesis from different types of non-meristematic explants of Passiflora cristalina. Leaf, hypocotyl, root segments, cotyledons, and endosperm of P. cristalina seeds were inoculated in Murashige and Skoog (MS)-basal medium, supplemented with different concentrations of 6-Benzyladenine (BA), Thidiazuron (TDZ), or Kinetin (KIN). BA was found to be the most efficient cytokinin in induction of de novo organogenesis from most the explants used in the study. The highest frequencies of adventitious bud formation in the hypocotyl and cotyledon explants were observed in medium supplemented with 1.0 mg L−1 BA. For leaf and endosperm segments, the best concentration was 2.0 mg L−1 BA; while for root segments, the highest mean values were observed with 1.0 mg L−1 KIN. The different morphogenetic responses obtained from each explant source were characterized using light microscopy. P. cristalina revealed a remarkable organogenic potential, with superior production of adventitious shoots compared with the other Passiflora species evaluated elsewhere. These results will be helpful to establish a reproducible and reliable micropropagation protocol, as well as to implement conservationist and biotechnological-based genetic breeding strategies for this wild Passiflora species.

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Acknowledgements

The authors would like to thank the CAPES and FAPEMAT for the financial support.

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Authors and Affiliations

  1. Programa de Pós Graduação em Genética e Melhoramento de Plantas, Universidade do Estado de Mato Grosso, Rod. MT 358, Km 7, Jardim Aeroporto, Tangará da Serra, Mato Grosso, 78300-000, Brazil

    Rodrigo Brito de Faria

  2. Departamento de Biologia, Instituto de Ciências Faculdade de Ciências Agrárias, Biológicas e da Saúde—FACABS, Universidade do Estado de Mato Grosso, Rod. MT 358, Km 7, Jardim Aeroporto, Caixa Postal 287, Tangará da Serra, Mato Grosso, 78300-000, Brazil

    Ilio Fealho de Carvalho & Maurecilne Lemes da Silva

  3. Departamento de Biologia, Faculdade de Ciências Biológicas e Agrárias, Universidade do Estado de Mato Grosso, Avenida Perimetral Rogério Silva, S/N, Bairro Flamboyant, Caixa Postal 324, Alta Floresta, Mato Grosso, 78580-000, Brazil

    Ana Aparecida Bandini Rossi

  4. Instituto de Ciências Biológicas, Departamento de Biologia, Universidade Federal de jIJuiz de Fora, MG, Rua José Lourenço Kelmer, s/n—Campus Universitário, Bairro: São Pedro Cidade/Estado, Juiz de Fora, Minas Gerais, 36036-900, Brazil

    Elyabe Monteiro de Matos

  5. Universidade Federal de Goiás—Regional Jataí, Unidade Acadêmica de Biociências, BR 364, Km 195 n° 3800—Campus Cidade Universitária, Jataí, GO, CEP: 75801-615, Brazil

    Diego Ismael Rocha

  6. Department of Biological Sciences, University of Rhode Island, 120 Flagg Road, Kingston, RI, 02881, USA

    Daniela Lopes Paim Pinto

  7. Departamento de Biologia Vegetal, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Viçosa, Minas Gerais, 36570-000, Brazil

    Wagner Campos Otoni

Authors
  1. Rodrigo Brito de Faria
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  2. Ilio Fealho de Carvalho
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  3. Ana Aparecida Bandini Rossi
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  4. Elyabe Monteiro de Matos
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  5. Diego Ismael Rocha
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  6. Daniela Lopes Paim Pinto
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  7. Wagner Campos Otoni
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  8. Maurecilne Lemes da Silva
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Corresponding author

Correspondence to Maurecilne Lemes da Silva.

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Editor: Ewen Mullins

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de Faria, R.B., de Carvalho, I.F., Rossi, A.A.B. et al. High responsiveness in de novo shoot organogenesis induction of Passiflora cristalina (Passifloraceae), a wild Amazonian passion fruit species. In Vitro Cell.Dev.Biol.-Plant 54, 166–174 (2018). https://doi.org/10.1007/s11627-017-9881-y

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