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Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants

  • Genetic Transformation and Hybridization
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Abstract

An efficient protocol for the Agrobacterium tumefaciens-mediated transformation of calla lily (Zantedeschia elliottiana (W. Wats.) Engl. cultivar ‘Florex Gold’) is described. Shoot basal discs were co-cultivated with A. tumefaciens C58C1 carrying a plasmid containing neomycin phosphotransferase (nptII) and plant ferredoxin-like protein (pflp) genes. After Agrobacterium co-cultivation, the shoot basal discs were exposed to 100 mg l−1 kanamycin for selection. Twenty-eight out of 260 discs (10.8%) were found to have survived and produced shoot clusters. Twenty-six of these were confirmed to contain the pflp transgene by PCR, ending up in 10% transformation efficiency. The disease resistance investigation revealed that 18 transgenic plants exhibited resistance to soft rot disease caused by Erwinia carotovora subsp. carotovora. The presence of pflp gene was demonstrated by PCR, and its accumulation and activity was confirmed by Western blot and disease resistance assay. This was the first report to show the successful transformation and resistance to a bacterial pathogen in Zantedeschia. The protocol is useful for the quality improvement of calla lily through genetic transformation.

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Abbreviations

AS:

Acetosyringone

DMSO:

Dimethylsulfoxid

ECC:

Erwinia carotovora subsp. carotovora

FG:

Florex Gold

HR:

Hypersensitive response

PFLP:

Plant ferredoxin-like protein

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Acknowledgements

We wish to express our gratitude to Dr. Wei-Chin Chang for critically reviewing this manuscript; Dr. Cheng-Hsien Chen for his kind guidance in molecular technology. We are also giving our deep appreciation to the Transgenic Plant Laboratory, Academic Sinica for providing experimental equipment and facilities. This work was supported by grants from the National Science Council of the Republic of China.

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Authors and Affiliations

  1. Institute of Plant and Microbial Biology, Academic Sinica, Nankang, Taipei, 11529, Taiwan, ROC

    Mei-Kuen Yip, Hsiang-En Huang, Shih-Hua Chiu, Yuh-Chih Tsai, Chin-I Lin & Teng-Yung Feng

  2. Department of Life Science, National University of Kaohsiung, Kaohsiung, 811, Taiwan, ROC

    Mang-Jye Ger

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  1. Mei-Kuen Yip
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  2. Hsiang-En Huang
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  3. Mang-Jye Ger
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  4. Shih-Hua Chiu
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  5. Yuh-Chih Tsai
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  6. Chin-I Lin
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  7. Teng-Yung Feng
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Correspondence to Teng-Yung Feng.

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Communicated by L. Peña

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Yip, MK., Huang, HE., Ger, MJ. et al. Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants. Plant Cell Rep 26, 449–457 (2007). https://doi.org/10.1007/s00299-006-0246-y

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  • DOI: https://doi.org/10.1007/s00299-006-0246-y

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