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Sugar-induced tolerance to the herbicide atrazine in Arabidopsis seedlings involves activation of oxidative and xenobiotic stress responses

  • Biotic and Abiotic Stress
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Abstract

Exogenous sucrose confers to Arabidopsis seedlings a very high level of tolerance to the herbicide atrazine that cannot be ascribed to photoheterotrophic growth. Important differences of atrazine tolerance between sucrose and glucose treatments showed that activation of chloroplast biogenesis per se could not account for induced tolerance. Sucrose-induced acquisition of defence mechanisms was shown by the gene expression pattern of a chloroplastic iron superoxide dismutase and by enhancement of whole-cell glucose-6-phosphate dehydrogenase activity. Activation of these defence mechanisms depended on both soluble sugar and atrazine. Moreover, acquisition of sucrose protection was shown to unmask atrazine-induced gene expression, such as that of a cytosolic glutathione-S-transferase, which remained otherwise cryptic because of the lethal effects of atrazine in the absence of soluble sugars.

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Abbreviations

Fe-SOD:

Iron-superoxide dismutase

FW:

Fresh weight

Glc:

Glucose

G6PDH:

Glucose-6-phosphate dehydrogenase

GST:

Glutathione-S-transfe-rase

MS:

Murashige and Skoog

PSII:

Photosystem II

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

Suc:

Sucrose

Ws:

Wassilewskija

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Acknowledgements

This work was supported in part by the programme Environnement-Vie-Société (CNRS, France). We thank the RIKEN Genomic Sciences Center (Japan) for providing cDNA clones.

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Authors and Affiliations

  1. Centre National de la Recherche Scientifique, Université de Rennes 1, UMR 6553 ECOBIO, Campus de Beaulieu, bâtiment 14A, F-35042, Rennes Cedex, France

    Cécile Sulmon, Gwenola Gouesbet, Abdelhak El Amrani & Ivan Couée

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  1. Cécile Sulmon
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  2. Gwenola Gouesbet
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  3. Abdelhak El Amrani
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  4. Ivan Couée
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Correspondence to Ivan Couée.

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Communicated by R. Schmidt

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Sulmon, C., Gouesbet, G., Amrani, A.E. et al. Sugar-induced tolerance to the herbicide atrazine in Arabidopsis seedlings involves activation of oxidative and xenobiotic stress responses. Plant Cell Rep 25, 489–498 (2006). https://doi.org/10.1007/s00299-005-0062-9

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