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Effects of boron deficiency in cell suspension cultures of Populus alba L.

  • Physiology and Biochemistry
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Abstract

Cell suspension cultures of Populus alba L. (original cells) require at least 10 μM boron for appropriate growth. Using original cells we established a cell line, T-5B, which can grow in a medium containing low levels of boron (5 μM). The level of boron localized in the cell walls of T-5B cells was one-half that found in the cell walls of original cells maintained in medium containing 100 μM boron, and the level of the rhamnogalacturonan II dimer, cross-linked by a borate ester, also decreased in the former. The sugar composition of whole cell walls of the T-5B cell line was similar that of the original cells, however pectic polysaccharides composed of arabinose or galacturonic acid were easily extracted from T-5B cell walls with 50 mM trans-1,2-cyclohexanediamine-N,N,N′,N′-tetraacetic acid. Our results suggest that boron deficiency causes a weakening of the interaction among pectic polysaccharides due to a decrease in boron-rhamnogalacturonanII cross-linkage.

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Abbreviations

Ara:

Arabinose

CDTA:

Trans-1,2-Cyclohexanediamine-N,N,N′,N′-tetraacetic acid monohydrate

Fuc:

Fucose

Gal:

Galactose

GalUA:

Galacturonic acid

Glc:

Glucose

GlcUA:

Glucuronic acid

Man:

Mannose

Rha:

Rhamnose

RG-II:

Rhamnogalacturonan II

Xyl:

Xylose

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Authors and Affiliations

  1. Department of Forest Chemistry, Forestry and Forest Products Research Institute, Matsunosato 1, Tukuba, Ibaraki, 305-8687, Japan

    Koichi Kakegawa & Tadashi Ishii

  2. Department of Agro-Environmental Research, National Agricultural Research Center for Kyushu Okinawa Region, Nishigoshi, Kumamoto, 861-1192, Japan

    Toshiro Matsunaga

Authors
  1. Koichi Kakegawa
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  2. Tadashi Ishii
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  3. Toshiro Matsunaga
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Correspondence to Koichi Kakegawa.

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Communicated by F. Sato

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Kakegawa, K., Ishii, T. & Matsunaga, T. Effects of boron deficiency in cell suspension cultures of Populus alba L.. Plant Cell Rep 23, 573–578 (2005). https://doi.org/10.1007/s00299-004-0878-8

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  • DOI: https://doi.org/10.1007/s00299-004-0878-8

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