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CO2 enrichment leads to altered cell wall composition in plants of Pfaffia glomerata (Spreng.) Pedersen (Amaranthaceae)

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Abstract

Pfaffia glomerata, popularly known as Brazilian-ginseng, stands out as a species of medicinal interest that has a high photoautotrophic potential for in vitro cultivation. This study aimed to analyze cell wall components of P. glomerata during in vitro cultivation in a CO2-enriched atmosphere. For this, P. glomerata plants were grown in MS medium without sucrose, in acrylic chambers with continuous forced air ventilation at 400 and 1000 μL L−1 CO2, and a control treatment with flasks put outside the chambers, without forced ventilation. The experiment was evaluated at 20, 30 and 40 days of cultivation, totaling nine treatments in a 3 × 3 factorial scheme (CO2 concentration × days), with 4 replications. Analyses of growth, photosynthesis and cell wall immunohistochemistry (using monoclonal antibodies JIM7, JIM13 and LM10) were done. The CO2 enrichment at the concentration of 1000 μL L−1 induced greater growth and accumulation of dry mass, in addition to increasing the photosynthetic rate. Immunohistochemistry analyses showed that the presence of homogalacturonan pectins detected by the JIM7 antibody decreased from 20 to 40 days, regardless of CO2 treatment. The deposition of heteroxylan and the JIM13 AGP epitope was detected exclusively in the secondary wall regions, with higher intensity in the treatment of 1000 µL L−1 CO2. This work opens new perspectives to understanding the dynamics between photoautotrophy and cell wall deposition in P. glomerata.

Key message

P. glomerata under elevated CO2 produces more pectic polysaccharides and hemicelluloses in the cell wall; increasing pectic polymers and xylans are associated with dry biomass gain in P. glomerata under high CO2.

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Data sharing not applicable to this article as no datasets were generated or analysed during the current study.

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Acknowledgements

The authors are also grateful to Dr. Paul Knox (University of Leeds, UK) for kindly providing the antibodies used and to the Núcleo de Microscopia e Microanálise (NMM) of the Universidade Federal de Viçosa for providing the facilities for the immunohistochemistry analysis. We would like to thank Editage (www.editage.com) for English language editing.

Funding

This work was supported by the Brazilian agencies Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, Belo Horizonte, MG, Brazil; Grants Nos. PRONEX-CAG-APQ-01036-09; CRA-APQ-01651-13; CRA-BPD-00046-14: APQ-00772-19; and CRA–RED-00053-16/REDE MINEIRA - Estresse em Plantas to WCO and CBB-BPD-00020-16 to DSB), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasília, DF, Brazil; Grant Finance Code 001) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasília, DF, Brazil: Grants Nos. MCT/CNPq 480675/2009-0; PQ 459.529/2014-5; and PQ 313901/2018-0 to WCO).

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Authors and Affiliations

  1. Departamento de Biologia Vegetal, Laboratório de Cultura de Tecidos/BIOAGRO, Universidade Federal de Viçosa, Campus Universitário, Avenida P. H. Rolfs s/n, Viçosa, MG, 36570-900, Brazil

    Eliza Louback, Tatiane Dulcineia Silva, Sérgio Heitor Sousa Felipe & Wagner Campos Otoni

  2. Departamento de Agricultura, Universidade Federal da Paraíba, Campus III, Bananeiras, PB, 58220-000, Brazil

    Diego Silva Batista

  3. Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-900, Viçosa, MG, Brazil

    Tiago Augusto Rodrigues Pereira

  4. Instituto Federal do Sertão Pernambucano, Petrolina, PE, 56302-970, Brazil

    Talita Cristina Mamedes-Rodrigues

  5. Departamento de Agronomia, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil

    Diego Ismael Rocha

  6. Vivitech Agrociências, Rua Mem de Sá 391, Marechal Cândido Rondon, PR, 85960-000, Brazil

    Douglas André Steinmacher

Authors
  1. Eliza Louback
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  2. Diego Silva Batista
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  3. Tiago Augusto Rodrigues Pereira
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  4. Talita Cristina Mamedes-Rodrigues
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  5. Tatiane Dulcineia Silva
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  6. Sérgio Heitor Sousa Felipe
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  7. Diego Ismael Rocha
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  8. Douglas André Steinmacher
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  9. Wagner Campos Otoni
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Contributions

EL, DSB, SHSF, TCM-Rand WCO designed the study; EL and TDS performed most of the experiments; EL, DSB, TDS and SHSF performed physiological and biochemical analyses; EL and TDS evaluated photosynthetic performance; EL and TARP performed histological studies; EL, TARP, DIR and DSB analyzed the data; EL, TARP, TCM-R, SHSF, DSB, DIR, PK, DS and WCO wrote the article with input from all other authors. All authors read and approved the manuscript.

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Correspondence to Wagner Campos Otoni.

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Communicated by Konstantin V. Kiselev.

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Louback, E., Batista, D.S., Pereira, T.A.R. et al. CO2 enrichment leads to altered cell wall composition in plants of Pfaffia glomerata (Spreng.) Pedersen (Amaranthaceae). Plant Cell Tiss Organ Cult 145, 603–613 (2021). https://doi.org/10.1007/s11240-021-02031-4

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