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Detection of the putative cis-region involved in the induction by a Pyricularia oryzae elicitor of the promoter of a gene encoding phenylalanine ammonia-lyase in rice

  • Genetics and Genomics
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Abstract

A rice PAL (phenylalanine ammonia-lyase) gene sequence (rPAL-P5), which is highly similar to and likely the same as a previously described rice ZB8PAL gene, including the 5′-upstream and exon I coding regions of PAL, was isolated using PCR amplification. The expression of several PALs, including rPAL-P5, was strongly induced following inoculation with Pyricularia oryzae or treatment with a P. oryzae elicitor. To identify the promoter region induced by the P. oryzae elicitor, we constructed and subsequently transformed rPAL-P5 promoter deletion series into rice calli using particle bombardment. Results from both elicitor-inducible reporter gene and gel mobility shift assays demonstrated that the sequence −349 to −256 of the rPAL-P5 promoter includes a cis-element involved in the induction of P. oryzae.

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Abbreviations

CTAB :

Cetyltrimethylammonium bromide

2,4-D :

2,4-Dichlorophenoxyacetic acid

GUS :

β-Glucuronidase

4-MU :

4-Methylumbelliferone

4-MUG :

4-Methylumbelliferyl glucuronide

NOS :

Nopaline synthase

PAL :

Phenylalanine ammonia-lyase

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Acknowledgements

We thank Prof. Youliang Peng of China Agricultural University for providing the rice seeds (Oryza sativa L. cv. Aizhixu) and the fungal strain of Pyricularia oryzae cv. 131. This work was supported by a grant from the National Natural Science Foundation (grant no. 39980003) and the National Key Basic Research “973” Program (grant no. G2000016204) of China.

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Authors and Affiliations

  1. National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, 100871, Beijing, P.R. China

    L. Wang, C. An, W. Qian, T. Liu, J. Li & Z. Chen

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  1. L. Wang
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  2. C. An
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  3. W. Qian
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  4. T. Liu
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  5. J. Li
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  6. Z. Chen
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Correspondence to C. An.

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Communicated by J.C. Register III

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Wang, L., An, C., Qian, W. et al. Detection of the putative cis-region involved in the induction by a Pyricularia oryzae elicitor of the promoter of a gene encoding phenylalanine ammonia-lyase in rice. Plant Cell Rep 22, 513–518 (2004). https://doi.org/10.1007/s00299-003-0717-3

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  • DOI: https://doi.org/10.1007/s00299-003-0717-3

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