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A simple method for enhancing paclitaxel release from Taxus canadensis cell suspension cultures utilizing cell wall digesting enzymes

  • Physiology and Biochemistry
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Abstract

Paclitaxel storage in Taxus suspension cell cultures was studied through the simple use of cell wall digesting enzymes. The application of cellulase (1%) and pectolyase (0.1%) to Taxus canadensis suspension cultures induced a significant increase in the paclitaxel present in the extracellular medium while maintaining membrane integrity, suggesting that paclitaxel is stored in the cell wall. The addition of cell wall digesting enzymes to a cell culture bioprocess may be an effective way of enhancing paclitaxel release to the extracellular medium and hence simplify product recovery.

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Fig. 1i–iv.

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Acknowledgements

This work was supported in part by grants from the National Cancer Institute (RO1 CA55138), National Science Foundation (BES-9625405 and BES-9984463) and the USDA.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Massachusetts, 159 Goessmann Laboratory, Amherst, MA 01003, USA

    S. C. Roberts & M. Naill

  2. United States Plant and Nutrition Laboratory, USDA, ARS, Tower Road, Ithaca, NY 14853, USA

    D. M. Gibson

  3. School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14850, USA

    M. L. Shuler

Authors
  1. S. C. Roberts
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  2. M. Naill
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  3. D. M. Gibson
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  4. M. L. Shuler
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Correspondence to S. C. Roberts.

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Communicated by K.K. Kamo

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Roberts, S.C., Naill, M., Gibson, D.M. et al. A simple method for enhancing paclitaxel release from Taxus canadensis cell suspension cultures utilizing cell wall digesting enzymes. Plant Cell Rep 21, 1217–1220 (2003). https://doi.org/10.1007/s00299-003-0575-z

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  • DOI: https://doi.org/10.1007/s00299-003-0575-z

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