Skip to main content
SpringerLink
Log in

Effect of elicitors, precursors and metabolic inhibitors on paclitaxel production by Taxus cuspidata cell culture

  • Original Paper
  • Published:
Journal of Forestry Research Aims and scope Submit manuscript

Abstract

In this study, using Taxus cuspidata as a raw material, we obtained stable high-yielding cell lines by subculturing and quantified paclitaxel content using ultrasonic extraction combined with TLC–UV spectrophotometry. In single factor and multiple factors tests to optimize design and study the effects of elicitors, precursors, and metabolic inhibitors on paclitaxel production by Taxus cuspidata cells, paclitaxel production reached 4.32 mg/L when 100 μmol/L methyl jasmonate, 20 mg/L salicylic acid, 400 mg/L phenylalanine and 2 mg/L gibberellin (GA3) were added to the culture medium of suspension cells. When adding metabolic adjustment factors on the 7th day of culture, extra- and intracellular paclitaxel production was the highest at 4.855 mg/L, paclitaxel release rate was 10.48 %, fresh mass and paclitaxel production of cell increased, respectively, by 6.08 and 11.57 %. By controlling the anabolism of paclitaxel, paclitaxel yield was significantly improved.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Gao MB, Li XT, Ruan CJ (2011) The callus induction of Taxus cuspidata. J Dali Natl Univ 13(3):256–259

    CAS  Google Scholar 

  • He DY, Yang F, Shi L, Liu TX, Zhang ZS (2010) Effects of hormones on taxol Taxus cuspidata nana accumulation in callus. J Southwest Agric Univ 23(2):538–541

    Google Scholar 

  • Kan CR, Cui JM, Wang JW, Wang WZ, Zhao YQ (2009) Determination of content of taxol in Taxus cuspidata extracting paclitaxel after residue. Chin Mod Tradit Med Compos 11(2):27–28

    CAS  Google Scholar 

  • Kwon IC, Yoo YJ, Lee JH, Hyun JO (1998) Enhancement of taxol production by in situ recovery of product. Process Biochem 33(7):701–707

    Article  CAS  Google Scholar 

  • Laskaris G, Bounkhay M, Theodoridis G, Vander Heijden R, Verpoorte R, Jaziri M (1999) Induction of geranyl–geranyl diphosphate synthase activity and taxane accumulation in Taxus baccata cell cultures after elicitation by methyl jasmonate. Plant Sci 147:1–8

    Article  CAS  Google Scholar 

  • Li H (2009) The technology of extraction of taxol from Taxus cuspidata leaves. Chin Pharm 18(19):44–45

    Google Scholar 

  • Li LQ, Fu CH, Yu LJ, Wang S, Yu LJ (2009) Effects of synchronization making taxol content in Taxus cells synergistic induction. Plant Physiol Commun 45(3):253–257

    CAS  Google Scholar 

  • Liu BY, Ye HC (1999) The research of biological production of taxol. Biotechnol Bull 34(7):5–7

    CAS  Google Scholar 

  • Mei XG, Zhou ZQ, Wu QJ (2001) The influence of elicitor, precursor and inhibitors on taxol biosynthesis. Biotechnology 5:11

    Google Scholar 

  • Qiao LJ, Man RL, Ni WD, Liang YH (2009) Research on extraction and purification of taxol in the branches of Taxus media. Chin J Chin Med 34(8):973–976

    CAS  Google Scholar 

  • Raskin I (1992) Salicylate, a new plant hormone. Plant Physiol 99:799–803

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Seki M, Ohzora C, Takeda M, Furusaki S (1997) Taxol (Paclitaxel) production using free and immobilized cells of Taxus cuspidata. Biotechnol Bioeng 53:214–219

    Article  CAS  PubMed  Google Scholar 

  • Tang ZH, Zhong WD (2006) Molecular mechanism of paclitaxel antitumor. Chin Clin Rehabil 10(27):125–127

    CAS  Google Scholar 

  • Yu XH, Shao JH, Yuan ZH, Zhao CH, Dai YQ (2013) The progress of plant cell engineering technology research on production of taxol. J Northwest 33(6):1279–1284

    CAS  Google Scholar 

  • Zhai HH, Fu YL (2010) The Taxus tissue culture. Dev For Sci Technol 24(4):98–100

    Google Scholar 

  • Zhai XX, Yang J, Li YY (2009) A new method making yew high yield cell line establishment and update fast. Biol Technol Commun 20(3):376–379

    CAS  Google Scholar 

  • Zhang XR (2009) Study on optimizing of environmental conditions of Taxus cuspidata cell culture to produce taxol. Master’s degree thesis, Jilin university, Jilin, pp 15–35

  • Zhao JP, Yang SS (2014) Establishment of cell suspension culture of Taxus media. J Northwest Agric For Univ (Nat Sci Ed) 42(1):189–195

    Google Scholar 

  • Zhong JJ (2002) Plant cell culture for production of paclitaxel and other taxanes. J Biosci Bioeng 94:591–599

    Article  CAS  PubMed  Google Scholar 

  • Zhou ZQ, Mei XG, Wu QJ (2002) Regulation of taxol production in cell suspension cultures of taxus chinensis by elicitors, precursors and inhibitors. Nat Prod Res Dev 14(2):19–21

    CAS  Google Scholar 

Download references

Acknowledgments

We thank College of Biological and Agricultural Engineering, Key Laboratory of Bionic Engineering (Ministry of Education) as well as College of Food Science and Engineering in Jilin University for support.

Author information

Authors and Affiliations

  1. College of Biological and Agricultural Engineering, Jilin University, Changchun, 130022, China

    Shujie Wang, Chun Li, Hujun Wang, Xiangmei Zhong, Jing Zhao & Yueying Tong

  2. Key Laboratory of Bionic Engineering (Ministry of Education), Jilin University, Changchun, 130022, China

    Shujie Wang & Yueying Tong

  3. College of Food Science and Engineering, Jilin University, Changchun, 130022, China

    Yajun Zhou

Authors
  1. Shujie Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hujun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiangmei Zhong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jing Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yueying Tong
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yajun Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yajun Zhou.

Additional information

Project funding: This work was supported by development plan project during “the 12th Five Year Plan” Nation Science and Technology in rural area (No. 2012AA10A506-04 and No. 2013AA103005-04), Changchun City science and technology development program (No. 2014174) and Changchun City science and technology support program (No. 2014NK002).

The online version is available at http://www.springerlink.com

Corresponding editor: Chai Ruihai

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, S., Li, C., Wang, H. et al. Effect of elicitors, precursors and metabolic inhibitors on paclitaxel production by Taxus cuspidata cell culture. J. For. Res. 27, 1257–1263 (2016). https://doi.org/10.1007/s11676-016-0217-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11676-016-0217-2

Keywords

Search

Navigation