Abstract
To compare the distribution of HLA-A, B, DRB1 and DQB1 alleles among Mexican patients with primary Sjögren Syndrome (pSS), secondary SS (sSS), connective tissue disease (CTD) without (w/o) SS and historical ethnically healthy controls. We included 28 pSS, 30 sSS, 96 CTD w/o SS patients and 234 controls. HLA-A, B, DRB1 and DQB1 were amplified and sequenced using the Allele SEQR Sequenced Based Typing Kits and analyzed on the ABI Prism*3130 DNA Analyzer using the Assign software. Gene frequencies were obtained by direct counting. Contingence tables of 2 × 2 were generated and analyzed by the Mantel–Haenzel χ 2 or Fisher’s test (EPIINFO program). We reported odds ratios (OR) and corrected p values. SS patients showed increased frequencies of A*68:01 and DRB1*14:06 alleles when compared to CTD w/o SS (OR 4.43, 95 % CI 1.35–14.14, p = 0.007 and OR 14, 95 % CI 1.68–116, p = 0.001, respectively) and a higher prevalence of DRB1*01:01 (OR 5.9, 95 % CI 2.13–16.56, p = 0.003) and HLA-B*35:01 (OR 3.70, 95 % CI 1.92–7.12, p = 0.004) when compared with controls. pSS patients had a higher frequency of DRB1*14:06 allele than sSS (OR 16, 95 % CI 1.59–390, p = 0.001). Anti-Ro/SSA positivity was associated with B*51:01 (OR 10.11, 95 % CI 1.09–245, p = 0.02) and DRB1*03:01 alleles (OR 4.26, 95 % CI 1.01–18.89, p = 0.029), whereas the A*01:01 allele was associated with anti-La/SSB positivity (OR 4.75, 95 % CI 1.32–16.92, p = 0.003). In our population, the DRB1*14:06 allele was associated with primary and secondary SS implying that both varieties bear a similar immunogenetic background.
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This work was supported by a grant from the Consejo Nacional de Ciencia y Tecnología and Departmental Funds.
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The authors have declared no conflicts of interest.
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Gabriela Hernández-Molina and Gilberto Vargas-Alarcón contributed equally to this manuscript.
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Hernández-Molina, G., Vargas-Alarcón, G., Rodríguez-Pérez, J.M. et al. High-resolution HLA analysis of primary and secondary Sjögren’s syndrome: a common immunogenetic background in Mexican patients. Rheumatol Int 35, 643–649 (2015). https://doi.org/10.1007/s00296-014-3143-7
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DOI: https://doi.org/10.1007/s00296-014-3143-7