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Association of tumor necrosis factor alpha and IL-10 promoter polymorphisms with rheumatoid arthritis in North Indian population

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Abstract

Rheumatoid arthritis is a chronic autoimmune disorder associated with altered expression of pro- and anti-inflammatory cytokines in the affected tissues. The aim of this study was to investigate the association between promoter polymorphisms of TNFα and IL-10 gene with susceptibility, age of disease onset and disease severity in North Indian patients with rheumatoid arthritis (RA). SNPs at position −308 and −863 of TNF gene and −819/−592 and −1082 position of IL-10 gene were determined in 222 patients and 208 healthy controls using RFLP or ARMS method. Polymorphism TNF −308A was less prevalent among the patients (1.7%) than controls (4.9%; p = 0.01, OR: 0.32, 95% CI: 0.13–0.76). Among female patients, IL-10 −592A allele associated with higher baseline disease activity scores (5.77 ± 1.99) than −592C (5.57 ± 1.19; p = 0.04). Female patients carrying allele A of TNFα −863 had earlier age of onset of RA (33.99 ± 9.6 years) than those with allele C (36.15 ± 11.21 years; p = 0.043). In conclusion, allele A at TNFα −308 locus provides protection against RA in North Indian population while another TNF allele A at −863 position had weak association with earlier onset of disease in female patients. On the other hand promoter polymorphisms of IL-10 did not affect susceptibility but polymorphism at −819/−592A was associated with higher disease activity scores at baseline.

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Acknowledgments

Deepali Gambhir is thankful to Department of Science and Technology, New Delhi, India for providing her fellowship and research grant for the project.

Conflict of interest statement

Authors DG, AL, AA, RM, SKM and SN have no conflict of interest.

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Authors and Affiliations

  1. Department of Immunology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India

    Deepali Gambhir, Able Lawrence, Amita Aggarwal, Ramnath Misra & Sita Naik

  2. Centre for Biomedical Research, Lucknow, India

    Sudhir Kumar Mandal

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  1. Deepali Gambhir
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  2. Able Lawrence
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  3. Amita Aggarwal
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Correspondence to Deepali Gambhir.

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Gambhir, D., Lawrence, A., Aggarwal, A. et al. Association of tumor necrosis factor alpha and IL-10 promoter polymorphisms with rheumatoid arthritis in North Indian population. Rheumatol Int 30, 1211–1217 (2010). https://doi.org/10.1007/s00296-009-1131-0

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  • DOI: https://doi.org/10.1007/s00296-009-1131-0

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