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Differential effect of IL-1β and TNF-α on the production of IL-6, IL-8 and PGE2 in fibroblast-like synoviocytes and THP-1 macrophages

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Abstract

Inflammation in the joint of rheumatoid arthritis is a complex immune reaction facilitated by various factors, such as cytokines, cells and hypoxia. Thus, we evaluated their relative capacity to produce proinflammatory mediators in response to IL-1β, TNF-α or IL-17 under hypoxia or normoxia in fibroblast-like synoviocytes (FLSs) and macrophages. The level of IL-6 expression was strongly increased in both FLSs and THP-1 macrophages in response to IL-1β and TNF-α, but the level by TNF-α was less than that by IL-1β. In contrast, the expression of IL-8 in both cell types was strongly stimulated by both IL-1β and TNF-α. In FLSs, PGE2 production increased only in response to IL-1β; and no effect was observed in THP-1 cells and TNF-α-stimulated FLSs. In addition, the production by IL-17 was extremely low when compared with those induced by IL-1β or TNF-α in FLSs and THP-1 cells. Hypoxia (2% O2) decreased IL-1β-stimulated production of PGE2, even though it increased the expression of mRNA and protein of COX-2. These results suggest that IL-1β and TNF-α differentially regulate gene expression in FLSs and macrophages under hypoxia or normoxia.

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Acknowledgments

This work was supported by a research grant from the Korean Ministry of Health and Welfare (03-PJ9-PG6-SO01-002).

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Authors and Affiliations

  1. East-West Neo Medical Center, East-West Bone and Joint Research Institute, Kyung Hee University, 149 Sangil-dong, Gangdong-gu, Seoul, Republic of Korea

    Hyun Mi Choi, Da Hee Oh, Jun Soo Bang, Hyung-In Yang, Myung Chul Yoo & Kyoung Soo Kim

  2. Department of Orthopedic Surgery, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, Gangdong-gu, Seoul, Republic of Korea

    Myung Chul Yoo

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  1. Hyun Mi Choi
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  2. Da Hee Oh
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Correspondence to Myung Chul Yoo or Kyoung Soo Kim.

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H. M. Choi and D. H. Oh contributed equally to this work.

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Choi, H.M., Oh, D.H., Bang, J.S. et al. Differential effect of IL-1β and TNF-α on the production of IL-6, IL-8 and PGE2 in fibroblast-like synoviocytes and THP-1 macrophages. Rheumatol Int 30, 1025–1033 (2010). https://doi.org/10.1007/s00296-009-1089-y

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