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Tumor necrosis factor alpha −308 polymorphism is associated with rheumatoid arthritis in Han population of Eastern China

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Abstract

The aim of this study was to investigate the association of single nucleotide polymorphisms (SNPs) in tumor necrosis factor alpha (TNFα) promoter with rheumatoid arthritis (RA) in Chinese Han population. Six SNPs at positions −238, −244, −308, −376, −857, −863 within TNFα promoter were genotyped in 367 unrelated RA patients and 271 healthy controls, using direct DNA sequencing method. Allelic, genotypic, and haplotypic associations of these SNPs with RA were examined. The frequencies of TNFα −308A allele and the haplotype GACC (in order of −238, −308, −857, −863) were significantly lower in RA patients when compared with healthy controls (P = 0.0046, P = 0.0045, respectively) but TNFα −308 polymorphism was not related to the clinical manifestations of RA patients. These results implied that TNFα gene itself or a gene in linkage disequilibrium with it might be associated with RA in Han population of Eastern China.

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Acknowledgments

This work were supported by the Nature Science Foundation of Shanghai (06ZR14107) and partially supported by grants from the National 863 Program.

We are grateful to Prof. Baohua Qian for providing healthy controls.

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Authors and Affiliations

  1. Department of Medical Genetics, Second Military Medical University, 800 Xiangyin Road, Shanghai, 200433, People’s Republic of China

    Ruiwen Chen, Meng Fang, Ke Lv, Ning Cheng & Shuhan Sun

  2. Department of Rheumatology and Immunology, Changhai Hospital, Second Military Medical University, Shanghai, People’s Republic of China

    Qing Cai

  3. Bio-X Life Science Research Center, Shanghai Jiao Tong University, PO Box 501, Hao Ran Building, 1954 Hua Shan Road, Shanghai, 200030, People’s Republic of China

    Shiwei Duan & Lin He

  4. State Key Lab of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, People’s Republic of China

    Daming Ren

  5. Guanghua Hospital, Shanghai, People’s Republic of China

    Jie Shen & Dongyi He

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  1. Ruiwen Chen
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  2. Meng Fang
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  3. Qing Cai
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  10. Lin He
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  11. Shuhan Sun
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Correspondence to Lin He or Shuhan Sun.

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R. Chen, M. Fang, Q. Cai are equally contributed to the work.

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Chen, R., Fang, M., Cai, Q. et al. Tumor necrosis factor alpha −308 polymorphism is associated with rheumatoid arthritis in Han population of Eastern China. Rheumatol Int 28, 121–126 (2007). https://doi.org/10.1007/s00296-007-0409-3

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  • DOI: https://doi.org/10.1007/s00296-007-0409-3

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