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Synthesis and characterization of bovine serum albumin-loaded microspheres based on star-shaped PLLA with a xylitol core and their drug release behaviors

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Abstract

The star-shaped poly(l-lactide) (s-PLLA) was synthesized via ring-opening polymerization of l-lactide, with xylitol as a multifunctional initiator and stannous octoate as a catalyst. The structures of s-PLLA were characterized by 1H NMR spectroscopy, while the molecular weight (M w) and polydispersity index were determined by gel permeation chromatography. Bovine serum albumin (BSA) loaded s-PLLA microspheres were fabricated by a water–oil–water (W 1/O/W 2) double-emulsion solvent evaporation method. The morphology, drug encapsulation efficiency (EE), and in vitro release behavior of the prepared microspheres were studied in detail. Results indicated that the average diameters of s-PLLA microspheres could be controlled between 7 and 15 µm by varying the s-PLLA’s concentration or M w, and the drug encapsulation efficiency of BSA-loaded microspheres were 10–42%. The in vitro study shows that the BSA-loaded microspheres prepared by high molecular weight s-PLLA had a slow cumulative release behavior in phosphate buffer saline (PBS, pH 7.4) and the release process followed the Baker–Lonsdale equation. Present work supposes that the synthesized s-PLLA is a good candidate for drug delivery.

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Acknowledgements

This work was financed by the National Natural Science Foundation of China (Grant No. 51403003), Anhui Provincial Natural Science Foundation (1508085QE105), Scientific Research Fund of Anhui Provincial Education Department (KJ2016A791, KJ2017A030), Postdoctoral Science Foundation of China (01001419) and the 211 Project of Anhui University.

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  1. Anhui Province Key Laboratory of Environment-Friendly Polymer Materials, College of Chemistry and Chemical Engineering, Anhui University, Hefei, 230601, China

    Amin Ding, Lijing Teng, Yifeng Zhou, Pengpeng Chen & Wangyan Nie

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  1. Amin Ding
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  2. Lijing Teng
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Correspondence to Wangyan Nie.

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Ding, A., Teng, L., Zhou, Y. et al. Synthesis and characterization of bovine serum albumin-loaded microspheres based on star-shaped PLLA with a xylitol core and their drug release behaviors. Polym. Bull. 75, 2917–2931 (2018). https://doi.org/10.1007/s00289-017-2197-6

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  • DOI: https://doi.org/10.1007/s00289-017-2197-6

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