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Preparation of polymeric microcapsules enclosing microbial cells by radical suspension polymerization via water-in-oil-in-water emulsion

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Abstract

Polymeric microcapsules enclosing Saccharomyces cerevisiae were prepared by radical suspension polymerization via water-in-oil-in-water emulsion. Trimethylolpropane trimethacrylate and 2,2′-azobis(4-methoxy-2,4-dimethylvaleronitrile) were used as monomer and radical initiator, respectively. A culture medium with suspended yeast cells, monomer solution with the dissolved radical initiator, and poly(vinyl alcohol) aqueous solution were used as inner aqueous phase, oil phase, and outer aqueous phase, respectively. The influence of microcapsule preparation parameters on the viability of encapsulated cells and encapsulation efficiency was investigated. The radical polymerization process did not cause significant damage to encapsulated yeast cells. Decreased weight ratio of aqueous phase to oil phase resulted in increased encapsulation efficiency of the cells. The diameter of the microcapsules could be controlled by varying the agitation rate.

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References

  1. Moslemy P, Guiot SR, Neufeld RJ (2002) Production of size-controlled gellan gum microbeads encapsulating gasoline-degrading bacteria. Enzyme Microb Technol 30:10–18

    Article  CAS  Google Scholar 

  2. Buyukgungor H (1992) Stability of Lactobacillus bulgaricus immobilized in kappa-carrageenan gels. J Chem Technol Biotechnol 53:173–175

    Article  CAS  Google Scholar 

  3. Talebnia F, Niklasson C, Taherzadeh MJ (2005) Ethanol production from glucose and dilute-acid hydrolyzates by encapsulated S. cerevisiae. Biotechnol Bioeng 90:345–353

    Article  CAS  Google Scholar 

  4. Uemura Y, Hamakawa N, Yoshizawa H, Ando H, Ijichi K, Hatate Y (2000) Effect of calcium alginate coating on the performance of immobilized yeast cells in calcium alginate beads. Chem Eng Commun 177:1–14

    Article  CAS  Google Scholar 

  5. Bowen GD, Rovira AD (1999) The rhizosphere and its management to improve plant growth. Adv Agron 66:1–102

    Article  Google Scholar 

  6. Cassidy MB, Lee H, Trevors JT (1996) Environmental applications of immobilized microbial cells: a review. J Ind Microbiol 16:79–101

    Article  CAS  Google Scholar 

  7. Guobin S, Jianmin X, Chen G, Huizhou L, Jiayong C (2005) Biodesulfurization using Pseudomonas delafieldii in magnetic polyvinyl alcohol beads. Lett Appl Microbiol 40:30–36

    Article  CAS  Google Scholar 

  8. Tenokuchi D, Yoshida M, Hatanaka C, Toorisaka E, Hatate Y (2006) Core-shell microbioreactor microencapsulated denitrifying bacteria for nitrate-nitrogen treatment. Polym Bull 56:275–284

    Article  CAS  Google Scholar 

  9. Laane C, Boeren S, Vos K, Veeger C (1987) Rules for optimization of biocatalysis in organic-solvents. Biotechnol Bioeng 30:81–87

    Article  CAS  Google Scholar 

  10. Kim JW, Ko JY, Jun JB, Chang IS, Kang HH, Suh KD (2003) Multihollow polymer microcapsules by water-in-oil-in-water emulsion polymerization: morphological study and entrapment characteristics. Colloid Polym Sci 281:157–163

    Article  CAS  Google Scholar 

  11. Ho CH, Chen SA, Amiridis MD, VanZee JW (1997) Dispersion polymerization of styrene in alcohol media: effect of initiator concentration, solvent polarity, and temperature on the rate of polymerization. J Polym Sci Pol Chem 35:2907–2915

    Article  CAS  Google Scholar 

  12. Kita Y, Sano A, Yamaguchi T, Oka M, Gotanda K, Matsugi M (1997) Additions of malononitrile radicals to alkenes under mild conditions using 2,2′-azobis-(2,4-dimethyl-4-methoxyvaleronitrile) (V-70) as an initiator. Tetrahedron Lett 38:3549–3552

    Article  CAS  Google Scholar 

  13. Takei T, Yoshida M, Hatate Y, Shiomori K, Kiyoyama S (2008) Lactic acid bacteria-enclosing poly(ε-caprolactone) microcapsules as soil bio-amendment. J Biosci Bioeng 106:268–272

    Article  CAS  Google Scholar 

  14. Naoshima Y, Nakamura A, Nishiyama T, Haramaki T, Mende M, Munakata Y (1989) Asymmetric bioreduction of ethyl 3-oxobutanoate by immobilized baker’s yeast entrapped in calcium alginate beads. Application of the immobilized biocatalyst to the synthesis of (5z,13s)-5-tetradecen-13-olide, aggregation pheromone of cryptolestes grain beetle. Chem Lett 6:1023–1026

    Article  Google Scholar 

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Acknowledgments

This research was partially supported by a Grant-in-Aid for the Global COE Program, “Science for Future Molecular Systems,” from the Ministry of Education, Culture, Science, Sports and Technology of Japan, and a grant from the Inamori Foundation.

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Authors and Affiliations

  1. Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan

    Takayuki Takei, Kaoru Ikeda, Hiroyuki Ijima & Koei Kawakami

  2. Department of Chemical Engineering, Graduate School of Engineering, Kagoshima University, Kagoshima, 890-0065, Japan

    Masahiro Yoshida & Yasuo Hatate

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  1. Takayuki Takei
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  2. Kaoru Ikeda
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  3. Hiroyuki Ijima
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  4. Koei Kawakami
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  5. Masahiro Yoshida
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  6. Yasuo Hatate
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Correspondence to Takayuki Takei.

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Takei, T., Ikeda, K., Ijima, H. et al. Preparation of polymeric microcapsules enclosing microbial cells by radical suspension polymerization via water-in-oil-in-water emulsion. Polym. Bull. 65, 283–291 (2010). https://doi.org/10.1007/s00289-010-0253-6

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  • DOI: https://doi.org/10.1007/s00289-010-0253-6

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