Summary
Droplets of water-in-oil-in-water (w/o/w) emulsion were prepared using sodium alginate solution with denitrifying bacteria (Paracoccus denitrificans IFO13301) and dichloromethane (DCM) with polymethylmethacrylate (PMMA). By phase-separation and solvent-evaporation these droplets could be formed into core-shell PMMA microencapsulated denitrifying bacteria (PMMA-MC) possessing a large single core and a highly porous wall. The average thickness of the PMMA shell was 30 μm and the denitrifying bacteria were incorporated in the inner core at a high density. The PMMA-MC completely reduced 20 mg/L nitrate-nitrogen (NO3-N) and the intermediate product, nitrite-nitrogen (NO2-N), to N2 in the presence of H2 using a batchwise method. Thus, incorporated denitrifying bacteria can be used to treat water polluted with NO3-N. The PMMA-MC can be used repeatedly, and the third denitrification experiment directly denitrified (NO3-N → N2) without the intermediate step (NO3-N → NO2-N). In addition, the PMMA-MC with decreased activity could be reactivated by incubating in a culture medium.
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Tenokuchi, D., Yoshida, M., Hatanaka, C. et al. Core-shell microbioreactor microencapsulated denitrifying bacteria for nitrate-nitrogen treatment. Polym. Bull. 56, 275–284 (2006). https://doi.org/10.1007/s00289-005-0478-y
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DOI: https://doi.org/10.1007/s00289-005-0478-y