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Isolation, Classification, and Growth-Promoting Effects of Pantoea sp. YSD J2 from the Aboveground Leaves of Cyperus Esculentus L. var. sativus

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Abstract

Plant growth-promoting (PGP) bacteria are an environmental-friendly alternative to chemical fertilizers for promoting plant development. We isolated and characterized a PGP endophyte, YSD J2, from the leaves of Cyperus esculentus L. var. sativus. Specific PGP characteristics of this strain, such as phosphate solubilization ability, potassium-dissolving ability, siderophore and indole-3-acetic acid (IAA) production, and salt tolerance, were determined in vitro. In addition, positive mutants were screened using the atmospheric and room-temperature plasma (ARTP) technology, with IAA level and organic phosphorus solubility as indices. Furthermore, the effect of the positive mutant on biomass production and antioxidant abilities of greengrocery seedling was evaluated and the genome was mined to explore the underlying mechanisms. The strain YSD J2 showed a good performance of PGP characteristics, such as the production of indole acetic acid and siderophores, solubilization ability of phosphate, and potassium-dissolving ability. It was recognized through 16S rRNA sequencing together with morphological and physiological tests and confirmed as Pantoea sp. The strain exposed to a mutation time of 125 s by ARTP had the highest IAA and organic phosphate (lecithin) concentrations of 10.34 mg/L and 16.52 mg/L, 42.06% and 34.15% higher than those of the initial strain. Inoculation of mutant strain YSD J2 significantly increased plant growth attributes and the activities of peroxidase and superoxide dismutase, respectively, but decreased the content of malondialdehyde significantly compared with the control. Furthermore, genome annotation and functional analysis were performed through whole-genome sequencing and PGP-related genes were identified. Our results indicated that the YSD J2 with PGP characteristics is a potential candidate for the development of biofertilizers.

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Abbreviations

C. esculentus L.:

Cyperus esculentus L. var. sativus Baeck

PGP:

Plant growth-promoting

IAA:

Indole-3-acetic acid

ARTP:

Atmospheric and room-temperature plasma

MR:

Methyl red

VP:

Voges–Proskauer

SOD:

Superoxide dismutase

POD:

Peroxidase

MDA:

Malondialdehyde

TCA:

Trichloroacetic acid

SMRT:

Single-molecule real-time

CDSs:

Coding sequences

GI:

Gene Island

KEGG:

Kyoto encyclopedia of genes and genomes

COG:

Clusters of Orthologous Group

NR:

Non-redundant protein

CAZy:

Carbohydrate-active enzymes

PHI:

Pathogen–host interactions

CARD:

Comprehensive antibiotic resistance database

NRPS:

Non-ribosomal peptide synthase

ANOVA:

Analysis of variance

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Funding

This study was supported by the Shanghai Agriculture Applied Technology Development Program (Grant No. 2020-2-1), the National Natural Science Foundation of China (31801511), the Shanghai Agricultural Science and Technology Innovation Action Plan No. 21N31900800, the Shanghai Natural Science Foundation (No. 19ZR1436800), the Shanghai Sailing Program (No. 20YF1443000), the Technology Support Project (No. KJZC202008), the Shanghai Fresh Corn Technology System Project (No.10 2017), and the SAAS Program for Excellent Research Team (No. 2022 (016)) for financial support to this study.

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  1. Saisai Wang and Jinbin Wang contributed equally to this work.

Authors and Affiliations

  1. School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Saisai Wang & Xueming Tang

  2. Biotechnology Research Institute Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China

    Jinbin Wang, Yifan Zhou, Yanna Huang & Xueming Tang

  3. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Xueming Tang

  4. Crops Ecological Environment Security Inspection and Supervision Center (Shanghai), Ministry of Agriculture and Rural Affairs, P.R.C., 2901 Beidi Road, Shanghai, 201106, China

    Jinbin Wang, Yifan Zhou & Yanna Huang

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  1. Saisai Wang
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Contributions

SSW, JBW, and YFZ analyzed the genomic data and were responsible for experiment. SSW and JBW wrote the manuscript. SSW, JBW, and YNH edited the manuscript. XMT conceived the experimental design and edited the manuscript.

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Correspondence to Yanna Huang or Xueming Tang.

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Wang, S., Wang, J., Zhou, Y. et al. Isolation, Classification, and Growth-Promoting Effects of Pantoea sp. YSD J2 from the Aboveground Leaves of Cyperus Esculentus L. var. sativus. Curr Microbiol 79, 66 (2022). https://doi.org/10.1007/s00284-021-02755-8

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