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Impact of Bacillus licheniformis SV1 Derived Glycolipid on Candida glabrata Biofilm

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Abstract

In the present investigation, we have evaluated the antibiofilm potential of Bacillus licheniformis SV1 derived glycolipid against C. glabrata biofilm. Impact of isolated glycolipid on the viability of C. glabrata and on inhibiting as well as eradicating ability of its biofilm were studied. Further, morphological alterations, reactive oxygen species generation (ROS) production and transcriptional expression of selected genes (RT-PCR) of C. glabrata in response with isolated glycolipid were studied. The isolated glycolipid (1.0 mg ml−1) inhibited and eradicated C. glabrata biofilm approximately 80% and 60%, respectively. FE-SEM images revealed glycolipid exposure results in architectural alteration and eradication of C. glabrata biofilm and ROS generation. Transcriptional studies of selected genes showed that the expression of AUS1, FKS1 and KRE1 were down-regulated, while that of ergosterol biosynthesis pathway and multidrug transporter increased, in the presence of glycolipid.

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Abbreviations

PI:

Propidium iodide

DCFDA:

2′,7′-Dichlorodihydrofluorescein diacetate

XTT:

2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt

ROS:

Reactive oxygen species

FE-SEM:

Field emission scanning electron microscopy

PBS:

Phosphate buffer saline

YPD:

Yeast peptone dextrose broth medium

FBS:

Foetal bovine serum

RPMI:

Roswell park memorial institute 1640 medium

TLC:

Thin-layer chromatography

HPLC:

High-performance liquid chromatography

FTIR:

Fourier transform infrared microscopy

GC-MS:

Gas chromatography–Mass spectroscopy

NMR:

Nuclear magnetic resonance

MTP:

Microtitre plate

BEC80 :

Biofilm inhibitory concentration

BEC60 :

Biofilm eradicating concentration

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Acknowledgements

SG is extremely thankful to the Department of Science and Technology, INSPIRE (DST, INSPIRE) Govt. of India (Grant No. IF130678) for providing financial support. PG acknowledges DBT-RAship, Department of Biotechnology, Govt. of India. The authors are also thankful to Institute Instrumentation Centre (IIC), IIT Roorkee and Department of Chemical Engineering, IIT Roorkee for providing the instrumentation facility.

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Authors and Affiliations

  1. Department of Biotechnology, National Institute of Technology Raipur, Raipur, 492001, Chhattisgarh, India

    Sonam Gupta

  2. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India

    Sonam Gupta, Payal Gupta & Vikas Pruthi

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  1. Sonam Gupta
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  2. Payal Gupta
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  3. Vikas Pruthi
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Contributions

SG and PG designed and performed the experiments under the supervision of VP. PG critically analysed the experimental data. SG wrote and revised the important intellectual content of the manuscript with the help of PG and VP along with the final approval of the version to be submitted for the publication.

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Correspondence to Sonam Gupta.

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Gupta, S., Gupta, P. & Pruthi, V. Impact of Bacillus licheniformis SV1 Derived Glycolipid on Candida glabrata Biofilm. Curr Microbiol 78, 1813–1822 (2021). https://doi.org/10.1007/s00284-021-02461-5

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