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In Vitro Apoptosis Induction in a Human Prostate Cancer Cell Line by Thermotolerant Glycolipid from Bacillus licheniformis SV1

  • Original Article
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Journal of Surfactants and Detergents

Abstract

A highly potent glycolipid was isolated from a crude oil-contaminated soil bacterium Bacillus licheniformis SV1 (NCBI GenBank Acc. No. KX130852) when grown on a modified mineral salt medium supplemented with 2% oleic acid. The maximum reduction in surface tension of cell-free broth from 71 ± 0.812 to 25.919 ± 0.984 mN/m with 89 ± 0.346% emulsification activity was recorded after 120 h of growth. Glycolipid was purified using chromatographic techniques and the presence of aliphatic chain (C–H stretch) and OH-band was revealed by NMR, GC–MS and FTIR analysis. Stability of glycolipid up to 250 °C and its complete decomposition at 507 °C was recorded by thermogravimetric analysis (TGA). MTT assay (IC50 = 0.473 ± 0.048 mg/ml) along with 4′,6-diamidino-2-phenylindole (DAPI) and acridine orange and ethidium bromide (AO/EtBr) staining validated that glycolipid induces cell apoptosis in human prostate cancer cell line (PC-3). Furthermore, potent anti-cancerous compounds such as 9-octadecanoic acid (22.55%), linoleic acid methyl ester (2.2%) and palmitic acid (1.18%) were also detected in the GC–MS spectra of the purified glycolipid.

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Abbreviations

MSM:

Mineral salt medium

AO/EtBr:

Acridine orange and ethidium bromide

DAPI:

4′,6-Diamidino-2-phenylindole

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Acknowledgements

Authors are highly grateful to the Department of Science and Technology, INSPIRE (DST, INSPIRE) Govt. of India (Grant No. IF130678) for providing financial assistance to carry out the present work. Authors are also thankful to Institute Instrumentation Centre (IIC), IIT Roorkee and Department of Chemical Engineering, IIT Roorkee for providing the instrumentation facility.

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India

    Sonam Gupta, Ritu Varshney, Parul A. Pruthi, Partha Roy & Vikas Pruthi

  2. Evolutionary and Medical Genetics Lab, CCMB, Hyderabad, India

    Rajan Kumar Jha

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  1. Sonam Gupta
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  2. Ritu Varshney
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  3. Rajan Kumar Jha
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  4. Parul A. Pruthi
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  5. Partha Roy
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  6. Vikas Pruthi
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Correspondence to Vikas Pruthi.

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Gupta, S., Varshney, R., Jha, R. et al. In Vitro Apoptosis Induction in a Human Prostate Cancer Cell Line by Thermotolerant Glycolipid from Bacillus licheniformis SV1. J Surfact Deterg 20, 1141–1151 (2017). https://doi.org/10.1007/s11743-017-1986-0

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  • DOI: https://doi.org/10.1007/s11743-017-1986-0

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