Abstract
Extracellular and cell-bound lipase-producing yeasts were isolated from the palm oil mill wastes and investigated for their potential uses as biocatalysts in biodiesel production. Twenty-six yeast strains were qualitatively screened as lipase producers. From those yeast strains, only six were selected and screened further for quantitative lipase production.The phylogenetic affiliations of the yeast strains were confirmed by investigating the D1/D2 domains of 26S rDNA and ITS1-5.8S-ITS2 molecular regions of the six yeast strains selected as potent lipase producers. The three yeast strains A4C, 18B, and 10F showed a close association with Magnusiomyces capitatus. Two yeast strains (17B and AgB) had a close relationship with Saprochaete clavata, whereas the strain AW2 was identified as Magnusiomyces spicifer. Three main catalytic activities of the yeast lipases were evaluated and Magnusiomyces capitatus A4C, among the selected lipase-producing yeasts, had the highest extracellular lipolytic enzyme activity (969 U/L) with the cell-bound lipolytic enzyme activity of 11.3 U/gdm. The maximum cell-bound lipolytic activity (12.4 U/gdm) was observed in the cell-bound lipase fraction produced by Magnusiomyces spicifer AW2 with an extracellular lipolytic enzyme activity of 886 U/L. Based on the specific hydrolytic enzymatic activities, the cell-bound lipases (CBLs) from the three yeast strains M. capitatus A4C, M. spicifer AW2, and Saprochaete clavata 17B were further investigated for biodiesel production. Among them, the CBL from M. spicifer AW2 synthesized the most FAME (fatty acid methyl esters) at 81.2% within 12 h indicating that it has potential for application in enzymatic biodiesel production.
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Abbreviations
- CBL:
-
Cell-bound lipase
- CBLs:
-
Cell-bound lipases
- CBM:
-
Cell biomass
- ECL:
-
Extracellular lipase
- FAME:
-
Fatty acid methyl ester
- LPM:
-
Lipase production medium
- RBM:
-
Rhodamine B medium
- TSM:
-
Tributyrin selective medium
- YM:
-
Yeast Malt
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Acknowledgements
The authors would like to thank the Higher Education Research Promotion and the Thailand’s Education Hub for Southern Regions of ASEAN Countries Project Office of the Higher Education Commission (Contract No. THE-AC 024/2015), Prince of Songkla University (Contract No. AGR6202081S), and the Thailand Research Fund (Grant No. RTA6280014) for funding and grant support. The authors would like to thank Associate Professor Seppo J. Karrila, Ph.D. (Chem Eng) for the professional English proofreading service.
Funding
This work was financially supported by the Higher Education Research Promotion and the Thailand’s Education Hub for Southern Regions of ASEAN Countries Project Office of the Higher Education Commission under Contract No. THE-AC 024/2015. In addition, the research was funded by Prince of Songkla University under Contract No. AGR6202081S. The second and third authors are supported by Thailand Research Fund under Grant No. RTA6280014.
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KAB: Conducting research work under advisor’s supervision, analyze, and interpret the data, drafting the manuscript. AU: Main advisor, design and monitor the experiment, analyze and interpret the data, discuss the results, prepare and edit the manuscript, submit and revise the manuscript. BC: Co-advisor, advising on research techniques, analyze, and interpret the data. FF: Analyze and interpret the data.
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Baloch, K.A., Upaichit, A., Cheirsilp, B. et al. The Occurrence of Triple Catalytic Characteristics of Yeast Lipases and Their Application Prospects in Biodiesel Production from Non-Edible Jatropha curcas Oil in a Solvent-Free System. Curr Microbiol 78, 1914–1925 (2021). https://doi.org/10.1007/s00284-021-02448-2
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DOI: https://doi.org/10.1007/s00284-021-02448-2