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Halomonas sedimenti sp. nov., a Halotolerant Bacterium Isolated from Deep-Sea Sediment of the Southwest Indian Ocean

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Abstract

A Gram-staining-negative, aerobic, flagellated, motile, rod-shaped, halophilic bacterium QX-2T was isolated from the deep-sea sediment of the Southwest Indian Ocean at a depth of 2699 m. Growth of the QX-2T bacteria was observed at 4–50 °C (optimum 30 °C), pH 5.0–12.0 (optimum pH 6.0) and 0%–30% NaCl (w/v) [optimum 4% (w/v)]. 16S rRNA gene sequencing revealed that strain QX-2T has the closest relationship with Halomonas titanicae DSM 22872T (98.2%). Phylogeny analysis classified the strain QX-2T into the genus Halomonas. The average nucleotide identity and DNA–DNA hybridization values between strain QX-2T and related type strains were lower than the currently accepted new species definition standards. Principal fatty acids (> 10%) determined were C16:0 (12.41%), C12:0-3OH (25.15%), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c, 11.55%) and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c, 16.06%). Identified polar lipids in strain QX-2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipid, unidentified aminophospholipid and five unidentified lipids (L1–L5). The main respiratory quinone was Q-9. The content of DNA G+C was determined to be 54.34 mol%. The results of phylogenetic analysis, phenotypic analysis and chemotaxonomic studies showed that strain QX-2T represents a novel species within the genus Halomonas, for which the name Halomonas sedimenti sp. nov. is proposed, with the type strain QX-2T (MCCC 1A17876T = KCTC 82199T).

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Abbreviations

MCCC:

Marine Culture Collection of China

CGMCC:

China General Microbiological Culture Collection Center

KCTC:

Korean Collection for Type Cultures

DSM:

Leibniz Institut Deutsche Sammlung von Mikroorganismen und Zellkulturen (German Collection of Microorganisms and Cell Cultures)

ANI:

Average nucleotide identity

DDH:

DNA–DNA hybridization

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Funding

This work was supported by the COMRA Project of China (DY135-B2-16), COMRA Project of China (DY135-B2-08) and National Basic Research Program of China (973 Program) (No.2015CB755901).

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  1. Xu Qiu and Libo Yu have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China

    Xu Qiu, Libo Yu, Xiaorong Cao, Huangming Wu, Guangxin Xu & Xixiang Tang

  2. School of Ocean Sciences, China University of Geosciences (Beijing), Beijing, 100083, China

    Xu Qiu

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Contributions

XQ performed the technical characterization on strain QX-2 and drafted the manuscript. LY, XC, HW and GX conceived the study and aided to draft the manuscript. XT conceived the study, participated in its design and coordination, and helped to draft the manuscript. All authors read and approved the final manuscript.

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Correspondence to Xixiang Tang.

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Qiu, X., Yu, L., Cao, X. et al. Halomonas sedimenti sp. nov., a Halotolerant Bacterium Isolated from Deep-Sea Sediment of the Southwest Indian Ocean. Curr Microbiol 78, 1662–1669 (2021). https://doi.org/10.1007/s00284-021-02425-9

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