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First Insight into the Probiotic Properties of Ten Streptococcus thermophilus Strains Based on In Vitro Conditions

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Abstract

The aim of this study was to evaluate probiotic properties of ten Streptococcus thermophilus strains (st1 to st10) isolated from pickles in China. These strains all had β-galactosidase activity, which laid foundation for studying their probiotic properties. In this study, the bile salt hydrolase activity, lysozyme resistance, tolerance to simulated gastric juice, bile salt tolerance, and bacterial adhesion capacity to the Caco-2 cells of these selected strains were detected in vitro conditions. The results indicated that the bile salt hydrolase activities of st2, st6, and st9 were higher than that for other strains. St10 showed the greatest lysozyme resistance (> 80% survival), followed by st9, st8, st7, st5, and st6. As for the tolerance to simulated gastric juice, st5 possessed the highest survival rate (35%), followed by st6 (30%). St6 was the best performer in both bile salt tolerance and bacterial adhesion capacity to the Caco-2 cells. The results of fluorescence microscope and electron microscope further confirmed previous studies and more intuitively demonstrated the st6 strain's tolerance to harsh environments. Overall, these strains were expected to possess beneficial properties and have the potentiality to be probiotics.

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Acknowledgements

This work was supported by the Ministry of Agriculture of Peoples’ Republic of China (Grant No. 201503142–10). Prof. Wu Ding of Northwest A&F University is acknowledged for providing Lactobacillus rhamnosus GG strains.

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  1. College of Food Science and Engineering, Northwest A & F University, Yangling, 712100, Shaanxi, China

    Jie Zhang, Miaomiao Liu, Junnan Xu, Yiman Qi, Ning Zhao & Mingtao Fan

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Zhang, J., Liu, M., Xu, J. et al. First Insight into the Probiotic Properties of Ten Streptococcus thermophilus Strains Based on In Vitro Conditions. Curr Microbiol 77, 343–352 (2020). https://doi.org/10.1007/s00284-019-01840-3

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