Abstract
Members of marine Actinobacteria have been highly regarded as potentially important sources of antimicrobial compounds. Here, we isolated a strain of Actinobacteria, SZJ61, and showed that it inhibits the in vitro growth of fungi pathogenic to plants. This new isolate was identified as Streptomyces luteoverticillatus by morphological, biochemical and genetic analyses. Antifungal compounds were isolated from S. luteoverticillatus strain SZJ61 and characterized as carbazomycin B by nuclear magnetic resonance spectra. We then sequenced the genome of the S. luteoverticillatus SZJ61 strain, which consists of only one 7,367,863 bp linear chromosome that has a G+C content of 72.05%. Thirty-five putative biosynthetic gene clusters for secondary metabolites, including a variety of bioactive products, were found. Mining of the genome sequence information revealed the putative biosynthetic gene cluster of carbazomycin B. This genomic information is valuable for interpreting the biosynthetic mechanisms of diverse bioactive compounds that have potential applications in the pharmaceutical industry.
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Acknowledgments
This work was financially supported by the National Key R&D Program of China (Grant No. 2017YFD0501800), the Innovation Team Project for Modern Agricultural Industrious Technology System of Shandong Province (Grant No. SDAIT-11-10), Major Scientific and Technological Innovation Project of Shandong Province (Grant No. 2017GGH5129), and Yantai Science and Technology Project (Grant No. 2017NC049).
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284_2019_1711_MOESM1_ESM.tif
Supplementary material 1—Antagonistic effect of S. luteoverticillatus SZJ61on the growth of plant pathogenic fungi. Aspergillus niger (A) and Fusarium oxysporum (B). (TIFF 226 kb)
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Feng, Z., Chen, G., Zhang, J. et al. Characterization and Complete Genome Analysis of the Carbazomycin B-Producing Strain Streptomyces luteoverticillatus SZJ61. Curr Microbiol 76, 982–987 (2019). https://doi.org/10.1007/s00284-019-01711-x
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DOI: https://doi.org/10.1007/s00284-019-01711-x