Skip to main content

Advertisement

SpringerLink
Log in

The Genome Sequence of a Novel Cyanophage S-B64 from the Yellow Sea, China

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

A novel cyanophage, S-B64, which can infect marine Synechococcus WH8102, was isolated from the coastal waters of the Yellow Sea using the liquid serial dilution method. Morphological study by transmission electron microscopy revealed that the cyanophage belongs to Podovirus. It’s genome, which was completely sequenced, contains a 151,867 bp DNA molecule with a G+C content of 41.78% and 186 potential open reading frames. The functions of the genes include cyanophage structure, cyanophage packaging, DNA replication and regulation. After primary characterization, it was found that the latent period is about 3 h, and it lysed after 8 h, the burst size is about 23 virions per cell. This information will provide an important benchmark for further research on the interaction between cyanophages and their hosts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Waterbury J et al (1986) Biological and ecological characterization of the marine unicellular cyanobacterium Synechococcus. Science 214:71–120

    Google Scholar 

  2. Roitman S et al (2018) Cyanophage-encoded lipid desaturases: oceanic distribution, diversity and function. ISME J 12(2):343–355

    Article  CAS  PubMed  Google Scholar 

  3. Murata K et al (2017) Visualizing adsorption of cyanophage P-SSP7 onto marine Prochlorococcus. Sci Rep 7:44176

    Article  PubMed  PubMed Central  Google Scholar 

  4. Lu J et al (2001) Distribution, isolation, host specificity, and diversity of cyanophages infecting marine Synechococcus spp. in river estuaries. Appl Environ Microbiol 67(7):3285–3290

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Suttle CA (2000) Cyanophages and their role in the ecology of cyanobacteria. In: Whitton BA, Potts M (eds) The ecology of cyanobacteria. Springer, Dordrecht, pp 563–589

    Google Scholar 

  6. Wilson WH, Joint IR, Carr NG et al (1993) Isolation and molecular characterization of five marine cyanophages propagated on Synechococcus sp. strain WH7803. Appl Environ Microbiol 59(11):3736–3743

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Labrie SJ et al (2013) Genomes of marine cyanopodoviruses reveal multiple origins of diversity. Environ Microbiol 15(5):1356–1376

    Article  CAS  PubMed  Google Scholar 

  8. Sullivan MB et al (2003) Cyanophages infecting the oceanic cyanobacterium Prochlorococcus. Nature 424(6952):1047–1051

    Article  CAS  PubMed  Google Scholar 

  9. Adriaenssens EM et al (2014) Using signature genes as tools to assess environmental viral ecology and diversity. Appl Environ Microbiol 80(15):4470–4480

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Lindell D et al (2007) Genome-wide expression dynamics of a marine virus and host reveal features of co-evolution. Nature 449(7158):83–86

    Article  CAS  PubMed  Google Scholar 

  11. Zhang Y et al (2013) The first isolation of a cyanophage-synechococcus system from the east China sea. Virol Sin 28(5):260–265

    Article  CAS  PubMed  Google Scholar 

  12. Wilhelm Steven W et al (2006) Marine and freshwater cyanophages in a laurentian great lake: evidence from infectivity assays and molecular analyses of g20 genes. Appl Environ Microbiol 72(7):4957–4963

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Deveau H et al (2006) Biodiversity and classification of lactococcal phages. Appl Environ Microbiol 72(6):4338–4346

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Goderdzishvili et al (2013) Isolation and characterisation of lytic bacteriophages of Klebsiella pneumoniae and Klebsiella oxytoca. Curr Microbiol 66(3):251–258

    Article  CAS  PubMed  Google Scholar 

  15. Waterbury JB et al (1993) Resistance to co-occurring phages enables marine Synechococcus communities to coexist with cyanophages abundant in seawater. Appl Environ Microbiol 59(10):3393–3399

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Xu Y et al (2016) Characterization, genome sequence, and analysis of Escherichia phage CICC 80001, a bacteriophage infecting an efficient l-aspartic acid producing Escherichia coli. Food Environ Virol 8(1):1–9

    Article  CAS  Google Scholar 

  17. Suttle CA et al (1993) Marine cyanophages infecting oceanic and coastal strains of Synechococcus: abundance, morphology, cross-infectivity and growth characteristics. Mar Ecol Prog 92(1-2):99–109

    Article  Google Scholar 

  18. Mann NH et al (2003) Phages of the marine cyanobacterial picophytoplankton. FEMS Microbiol Rev 27(1):17–34

    Article  CAS  PubMed  Google Scholar 

  19. Lindell D et al (2005) Photosynthesis genes in marine viruses yield proteins during host infection. Nature 438(7064):86

    Article  CAS  PubMed  Google Scholar 

  20. Sabehi G et al (2012) A novel lineage of myoviruses infecting cyanobacteria is widespread in the oceans. Proc Natl Acad Sci USA 109(6):2037–2042

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This study was support by the fund from the National Natural Science Foundation of China (No. 31500339, 41676178 and 41076088), and the Marine Scientific and Technological Innovation Project Financially Supported by Pilot National Laboratory for Marine Science and Technology (Qingdao) (2018SDKJ0406-6, 2016ASKJ14), at the same time, thanks are due to the Fundamental Research Funds for the Central University of Ocean University of China (Grant nos. 201812002, 201762017, 201562018).

Author information

Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Siyuan You, Min Wang, Yong Jiang, Tong Jiang, Yundan Liu, Xinxin Liu, Qingwei Yang & Hongbing Shao

  2. Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China

    Min Wang & Yong Jiang

  3. Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao, 266003, China

    Min Wang & Yong Jiang

Authors
  1. Siyuan You
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Min Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yong Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tong Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yundan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xinxin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Qingwei Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hongbing Shao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Min Wang or Yong Jiang.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

You, S., Wang, M., Jiang, Y. et al. The Genome Sequence of a Novel Cyanophage S-B64 from the Yellow Sea, China. Curr Microbiol 76, 681–686 (2019). https://doi.org/10.1007/s00284-019-01680-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-019-01680-1

Search

Navigation