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Isolation and Complete Genome Sequence of a Novel Cyanophage S-B68

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Abstract

Cyanophages, which play a significant role in food web and global biochemical cycle, are one of the main causes of microbial death in aquatic environment. A novel cyanophage S-B68 was isolated from the surface water of the Bohai Sea, northern China. It can infect marine Synechococcus sp. (strain WH7803). The transmission electron microscopy results demonstrate that this cyanophage has an icosahedral head (51 nm in diameter) and a long tail (110 nm in length) and belongs to family Siphophages. The complete genome sequence of cyanophage S-B68 contains a linear, double-stranded 163,982 bp DNA molecule with a 51.7% G+C content. Except for four tRNAs, the genome contains 229 open reading frames (ORFs) which were grouped into six functional modules as follows: structure, hypothetical protein, DNA replication and expression, lysis, packaging, and some additional functions. It was found in one-step growth curve that the latent period of the S-B68 was about 49 h after infection with Synechococcus, and then it entered the rising period, and tended to stable after 61 h. Using the BLASTN tool in the NCBI database for genome comparison, there was no significant similarity between S-B68 and other known cyanophages. Present study adds a novel Siphoviridae genome to marine cyanophage dataset and provides useful basic information for its further research.

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Acknowledgements

We are grateful to the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (Nos. 2018SDKJ0406-6 and 2018SDKJ0104-4), the National Key Research and Development Program of China (Nos. 2018YFC1406704 and 2017YFA0603200), the National Natural Science Foundation of China (Nos. 31500339 and 41076088), and Fundamental Research Funds from the Central University of Ocean University of China (Nos. 201812002, 201762017 and 201562018). We appreciate the captain and crews of the RV ‘Dongfanghong 2′ and thank Prof. Hongbin Liu for providing the synechococcus host. The authors would like to thank the editor and anonymous reviewers for their constructive comments.

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Liyang Huang, Qian Liu, Xinxin Liu, Qi Wang, Qiannan Zhao, Min Wang, Hongbing Shao, Cui Guo, Yantao Liang & Yong Jiang

  2. Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China

    Min Wang, Cui Guo, Yantao Liang & Yong Jiang

  3. Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao, 266003, China

    Min Wang, Cui Guo, Yantao Liang & Yong Jiang

Authors
  1. Liyang Huang
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  2. Qian Liu
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  3. Xinxin Liu
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  4. Qi Wang
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  5. Qiannan Zhao
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  6. Min Wang
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  7. Hongbing Shao
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  8. Cui Guo
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  9. Yantao Liang
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  10. Yong Jiang
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Contributions

YJ participated in experimental design; LH, QL, XL and QZ were involved in the discussion; LH performed the main experiments and drafted the manuscript; MW, HS, CG, YL and YJ revised the manuscript.

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Correspondence to Yong Jiang.

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Huang, L., Liu, Q., Liu, X. et al. Isolation and Complete Genome Sequence of a Novel Cyanophage S-B68. Curr Microbiol 77, 2385–2390 (2020). https://doi.org/10.1007/s00284-020-02045-9

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  • DOI: https://doi.org/10.1007/s00284-020-02045-9

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