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Transcriptomic Analysis of the Swarm Motility Phenotype of Salmonella enterica Serovar Typhimurium Mutant Defective in Periplasmic Glucan Synthesis

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Abstract

Movement of food-borne pathogens on moist surfaces enables them to migrate towards more favorable niches and facilitate their survival for extended periods of time. Salmonella enterica serovar Typhimurium mutants defective in Osmoregulated periplasmic glucans (OPG) synthesis are unable to exhibit motility on moist surfaces (swarming); however, their mobility in liquid (swim motility) remains unaffected. In order to understand the role of OPG in swarm motility, transcriptomic analysis was performed using cells growing on a moist agar surface. In opgGH deletion mutant, lack of OPG significantly altered transcription of 1039 genes out of total 4712 genes (22%). Introduction of a plasmid-borne copy of opgGH into opgGH deletion mutant restored normal expression of all but 30 genes, indicating a wide-range influence of OPG on gene expression under swarm motility condition. Major pathways that were differentially expressed in opgGH mutants were motility, virulence and invasion, and genes related to the secondary messenger molecule, cyclic di-GMP. These observations provide insights and help explain the pleiotropic nature of OPG mutants such as sub-optimal virulence and competitive organ colonization in mice, biofilm formation, and sensitivity towards detergent stress.

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Acknowledgement

We acknowledge skillful technical assistance of Ms. Nadia George.

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Authors and Affiliations

  1. Environmental, Microbial, & Food Safety Laboratory, Beltsville Agriculture Research Center, USDA-ARS (USDA/ARS/EMFSL), 10300 Baltimore Ave., B173, Rm. 204, BARC-E, Beltsville, MD, 20705, USA

    Arvind A. Bhagwat

  2. National Institutes of Health Library, Division of Library Services, Office of Research Services, National Institute of Health, Building 10, Bethesda, MD, 20892, USA

    Lynn Young & Medha Bhagwat

  3. Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, USDA-ARS, Beltsville, MD, 20705, USA

    Allen D. Smith

Authors
  1. Arvind A. Bhagwat
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  2. Lynn Young
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  4. Medha Bhagwat
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Correspondence to Arvind A. Bhagwat or Allen D. Smith.

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Bhagwat, A.A., Young, L., Smith, A.D. et al. Transcriptomic Analysis of the Swarm Motility Phenotype of Salmonella enterica Serovar Typhimurium Mutant Defective in Periplasmic Glucan Synthesis. Curr Microbiol 74, 1005–1014 (2017). https://doi.org/10.1007/s00284-017-1267-1

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