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ClpP Affects Biofilm Formation of Streptococcus mutans Differently in the Presence of Cariogenic Carbohydrates Through Regulating gtfBC and ftf

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Abstract

The abilities to form biofilms on teeth surface and to metabolize a wide range of carbohydrates are key virulence attributes of Streptococcus mutans. ClpP has been proved to play an important role in biofilm development in streptococci. Here we demonstrated that ClpP was involved in biofilm formation of S. mutans. ClpP inactivation resulted in enhanced biofilm formation or initial cell adherence in broth supplemented with sucrose, while reduced in broth supplemented with glucose or fructose. Our results also indicated that the enhanced capacities of biofilm formation and initial cell adherence were achieved through regulating the expression of a number of extracellular sucrose-metabolizing enzymes, such as glucosyltransferases (GTFB and GTFC) at early-exponential growth phase and fructosyltransferase at late-exponential growth phase in the presence of sucrose.

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Acknowledgments

The authors would like to thank S. Hou for technical assistance and Q. Xu and H. Rao for critically reading the manuscript. This work was supported through funding from the National Natural Science Foundation of China (No. 81000762), the Natural Science Foundation (No. 2010D018) of Fujian Province, China.

Conflict of interest

The authors have no conflicts of interest to declare.

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    Authors and Affiliations

    1. Department of Clinical Laboratory, The First Affiliated Hospital of Xiamen University, No. 55 Zhenhai Road, Xiamen, 361003, China

      Jia-qin Zhang, Xiu-yu Song, Xiao-bo Ma & Yuan-xun Zhao

    2. Nosocomial Infection Control Center of Xiamen, Xiamen, 361003, China

      Jia-qin Zhang & Shi-yang Zhang

    3. Department of Nephrology, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, China

      Xiang-hua Hou

    4. Department of Nosocomial Infection Control, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, China

      Shi-yang Zhang

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    1. Jia-qin Zhang
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    Correspondence to Jia-qin Zhang.

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    Jia-qin Zhang and Xiang-hua Hou have contributed equally.

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    Zhang, Jq., Hou, Xh., Song, Xy. et al. ClpP Affects Biofilm Formation of Streptococcus mutans Differently in the Presence of Cariogenic Carbohydrates Through Regulating gtfBC and ftf . Curr Microbiol 70, 716–723 (2015). https://doi.org/10.1007/s00284-015-0779-9

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    • DOI: https://doi.org/10.1007/s00284-015-0779-9

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