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Two Bacterial Group II Phosphopantetheinyl Transferases Involved in Both Primary Metabolism and Secondary Metabolism

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Abstract

It is known that bacterial group II phosphopantetheinyl transferases (PPTases) usually phosphopantetheinylate acyl carrier proteins (ACPs) involved in the secondary metabolism. For example, a bacterial group II PPTase SchPPT has been known to phosphopantetheinylate only ACPs involved in secondary metabolism, such as scn ACP0-2 and scn ACP7. In this study, we found two bacterial group II PPTases, Hppt and Sppt, could phosphopantetheinylate not only scn ACP0-2 and scn ACP7, but also sch FAS ACP, an ACP involved in primary metabolism. Swapping of the N terminus and C terminus of PPTases showed that (i) both the hybrids Hppt-Sppt and Sppt-Hppt could phosphopantetheinylate sch FAS ACP but not scn ACP0-2; (ii) both the hybrids Sppt-SchPPT and SchPPT-Sppt lost abilities to phosphopantetheinylate sch FAS ACP and scn ACP0-2. Hppt and Sppt represent group II PPTases which phosphopantetheinylate both ACPs involved in primary metabolism and ACPs involved in secondary metabolism.

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Acknowledgments

This work was supported by Zhejiang Provincial Natural Science Foundation of China LZ12C01001, National Natural Science Foundation of China 31200600, National High Technology Research & Development Program of China (863 Program) 2012AA02A706 and 2012AA022107, and National Key Technologies R & D Program of China 2011BAD23B05-2. The authors of this manuscript have no conflict of interest to declare.

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China

    Yue-Yue Wang, Xiao-Sheng Zhang, Ni-Ni Ren, Yuan-Yang Guo, Xin-Hang Jiang, Hui Jiang & Yong-Quan Li

  2. Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, Zhejiang, China

    Hui Jiang & Yong-Quan Li

  3. Department of Bioengineering, Zhejiang Gongshang University, Hangzhou, Zhejiang, China

    Yu-Dong Li

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  1. Yue-Yue Wang
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  3. Ni-Ni Ren
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  4. Yuan-Yang Guo
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  6. Hui Jiang
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Correspondence to Hui Jiang.

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Wang, YY., Zhang, XS., Ren, NN. et al. Two Bacterial Group II Phosphopantetheinyl Transferases Involved in Both Primary Metabolism and Secondary Metabolism. Curr Microbiol 70, 390–397 (2015). https://doi.org/10.1007/s00284-014-0735-0

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  • DOI: https://doi.org/10.1007/s00284-014-0735-0

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