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Production and in Vivo Antioxidant Activity of Zn, Ge, Se-enriched Mycelia by Cordyceps sinensis SU-01

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Abstract

Cordyceps sinensis, a traditionally edible and medicinal fungus in China, cannot be artificially solid-cultured. Zinc (Zn), germanium (Ge), and selenium (Se) are the essential trace elements for human body. In this work, C. sinensis SU-01 was cultivated in liquid medium simultaneously containing Zn, Ge, and Se. The bioactive ingredients and in vivo antioxidant activities of Zn, Ge, Se-enriched mycelia (ZGSM) of C. sinensis SU-01 were investigated. Under the determined conditions, the Zn, Ge, and Se contents of ZGSM were 2543.16 ± 158.92, 1873.85 ± 81.82, and 1260.16 ± 107.12 μg/g, respectively. The optimal concentrations of Zn, Ge, and Se had a positive effect on biosynthesis of protein, polysaccharide, cordycepic acid, and amino acids. The activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) of mice blood were 3.72 ± 0.15 and 28.74 ± 2.53 % higher than that of control, respectively, and the content of malondialdehyde (MDA) was 41.01 ± 3.66 % lower than that of control.

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References

  1. Bayer WF, Fridovich JL (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161:559–566

    Article  Google Scholar 

  2. Behne D, Kyriakopoulos A, Kalcklosch M, Weiss-Nowak C, Pfeifer H, Gessner H (1997) Two new selenoproteins found in the prostatic glandular epithelium and in the spermatid nuclei. Biomed Environ Sci 10:340–345

    CAS  PubMed  Google Scholar 

  3. Berry MJ, Banu L, Chen Y, Mandel SJ, Kieffer JD, Harney J (1991) Recognition of UGA as a selenocysteine codon in type I deiodinase requires sequences in the 3′-untranslated region. Nature 353:273–276

    Article  CAS  PubMed  Google Scholar 

  4. Chaplin MF, Kennedy JF (1994) Carbohydrate analysis, a practical approach. New IRL Press Ltd, York

    Google Scholar 

  5. Chen BQ (1999) Nutrition and food hygiene. People’s Medical Publishing House, Beijing

    Google Scholar 

  6. Chen SL, Xu ZH, Tao WY, Gu WY (2000) Germanium accumulation of Grifola frondosa. J Wuxi Univ Light Ind 19:248–254

    CAS  Google Scholar 

  7. Dang JZ, Sun HY, Xu BQ, Feng LX (2005) Determination of SOD and amino acids in Ge-enriched mycelia of Ganoderma lucidum. Li Shizhen Med Mat Med Res 16:1221–1222

    Google Scholar 

  8. Deng BW, Chen WQ (2000) Study on accumulation of selenium in Polystictus versicolor in submerged culture. Amino Acid Biol Res 22:21–24

    CAS  Google Scholar 

  9. Deng BW, Chen WQ (2002) Study on accumulation of Zinc (Zn) in Polyporus umbellatein in liquid culture. Edible Fungi China 22:33–34

    Google Scholar 

  10. Fan ZH, Tang WH, Lin L (2007) Determination of adenosine content in zymolytic powder of aweto by HPLC. Food Sci Technol 97:94–97

    Google Scholar 

  11. Fang YZ, Zheng RL (2002) The theory and application of free radical biology. Science Press, Beijing

    Google Scholar 

  12. Ge KY, Chang SY (1999) Dietary intake of micronutrients of Chinese inhabitants. Acta Nutr Sin 21:322–327

    CAS  Google Scholar 

  13. Hu GY, Li WW, Wang YJ (2007) A preliminary study on the polysaccharide contents in Se-accumulated Flammulina velutipes mycelia. Acta Edulis Fungi 7:11–14

    Google Scholar 

  14. Husain K, Somani SM, Boley TM, Hazelrigg SR (2003) Interaction of physical training and chronic nitroglycerin treatment on blood pressure and plasma oxidant/antioxidant systems in rats. Mol Cell Biochem 247:37–44

    Article  CAS  PubMed  Google Scholar 

  15. Jiang S, Xing AL (2006) Selenium enrichment by Trametes robiniphila in liquid culture. Edible Fungi 1:9–11

    Google Scholar 

  16. Landry J, Delhaye S, Jones DG (1992) Determination of tryptophan in feedstuffs: comparison of two methods of hydrolysis prior to HPLC analysis. J Sci Food Agric 58:439–441

    Article  CAS  Google Scholar 

  17. Li XQ, Bao TT, Wang Y (1999) Effects on determining mannitol content of mycelia of Cordyceps sinensis by different culture condition. Zhong Cao Yao 30:9–21

    Google Scholar 

  18. Liu BR, Wen HX, Xiao HD (2007) Screening on selenium-rich strains of Pleurotus ostreatus. Edible Fungi China 26:14–16

    Google Scholar 

  19. Miao JZ, Tu BJ, Lv ZQ (2007) Studies on bioenrichment of Se, Zn with Ganoderma lucidum by submerged culture. Food Res Dev 28:53–55

    Google Scholar 

  20. Niki E, Yoshida Y, Saito Y, Noguchi N (2005) Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochem Biophys Res Commun 338:668–676

    Article  CAS  PubMed  Google Scholar 

  21. Pallud S, Lennon AM, Ramauge M, Gavaret JM, Croteau W, Pierre M (1997) Expression of the type II iodothyronine deiodinase in cultured rat astrocytes is selenium dependent. J Biol Chem 272:104–110

    Article  Google Scholar 

  22. Park NS, Lee KS, Sohn HD, Kim DH, Lee SM, Park E (2005) Molecular cloning, expression, and characterization of the Cu, Zn superoxide dismutase (SOD1) gene from the entomopathogenic fungus Cordyceps militaris. Mycologia 97:130–138

    Article  CAS  PubMed  Google Scholar 

  23. Rabinovich M, Figlas D, Delmastro S, Curvetto N (2007) Copper- and zinc-enriched mycelium of Agaricus blazei Murrill: bioaccumulation and bioavailability. J Med Food 10:175–183

    Article  CAS  PubMed  Google Scholar 

  24. Ramauge M, Pallud S, Esfandiari A, Gavaret J, Lennon A, Pierre M (1996) Evidence the type III iodothyronine deiodinase in rat astrocyte is a selenoprotein. Endocrinology 137:21–25

    Google Scholar 

  25. Rong ZX, Liu HZ, Bao JQ (1994) Micromethod of glutathione peroxidase activity of the blood in mice. Prog Biochem Biophys 21:62–70

    Google Scholar 

  26. Serafin Muñoz AH, Kubachka K, Wrobel K, Gutierrez Corona JF, Yathavakilla SK, Caruso JA (2006) Se-enriched mycelia of Pleurotus ostreatus: distribution of selenium in cell walls and cell membranes/cytosol. J Agric Food Chem 54:3440–3444

    Article  PubMed  Google Scholar 

  27. Shao CG, Wang YP, Zhao LX (2008) Optimization experiments of fermentation conditions on Lentinus edodes selenium-enriched liquid. China Med Her 5:23–24

    Google Scholar 

  28. Shen HS, Chen JC, Tang BS, Li YB, Wu L (2009) Mycelial growth and selenium accumulation in Na2SeO3-supplemented cultures of Pleurotus geesteranus. Acta Edulis Fungi 16:35–38

    Google Scholar 

  29. Song Z, Jia L, Xu F, Meng FY, Deng P, Fan KM (2009) Characteristics of Se-enriched mycelia by Stropharia rugoso-annulata and its antioxidant activities in vivo. Biol Trace Elem Res 131:81–89

    Article  CAS  PubMed  Google Scholar 

  30. Tamura T, Stadtman TC (1996) A new selenoprotein from human lung adenocarcinoma cells: purification, properties, and thioredoxin reductase activity. P Natl Acad Sci USA 93:6–11

    Article  Google Scholar 

  31. Tang QY, Zhou YF, Qin EH, Yang L (2007) Function of Se for Armillaria mellea and accumulation of Se in polysaccharide. Food and Ferment Ind 33:96–98

    CAS  Google Scholar 

  32. Turlo J, Gutkowska B, Kalucka M, Bujak M (2007) Accumulation of zinc by the Lentinus edodes (Berk.) mycelium cultivated in submerged culture. Acta Pol Pharmacol 4:45–51

    Google Scholar 

  33. Ursini F, Maiorino M, Brigelius-Flohe R, Aumann KD, Roveri A, Schomburg D (1995) Diversity of glutathione peroxidases. Method Enzymol 252:38–53

    Article  CAS  Google Scholar 

  34. Wang GL, Shang DJ, Yang WX (2007) Study on the nutritional components and antioxidant of Ganoderma lucidum rich in selenium. Acta Nutr Sin 23:73–75

    Google Scholar 

  35. Wang T, Li W, Chen HW, Yu ZL (2009) Screening of Ge-enriched Cordyceps militaris strains by ion beam implantation. Sci Technol Food Ind 30:68–73

    Google Scholar 

  36. Wang X (1996) Trace element in life science. Metrology Press, Beijing

    Google Scholar 

  37. Yang KD, Zhang TB, Wang AG, Huang JM (2003) Trace elements and Health. Science Press, Beijing

    Google Scholar 

  38. Yang SQ, Wang CQ (1998) The first report on liquid cultivation of Cordyceps sinensis. Edible Fungi 18:28–29

    Google Scholar 

  39. Yu J, Cui PJ, Chen MZ, Zhong MQ, Cui SC, Wu QY, Zhou JY (2009) Selenium-enriched fermentation mycelia of Coprinus comatus enhance the antihyper-glycemia and antioxidant activities in alloxan-induced diabetic mice. Mycosystema 28:120–128

    Google Scholar 

  40. Zhang H, Mu ZB, Xu LM, Xu GF, Liu M, Shan AS (2009) Dietary lipid level induced antioxidant response in Manchurian trout, Brachymystax lenok (Pallas) larvae. Lipids 44:643–654

    Article  CAS  PubMed  Google Scholar 

  41. Zhang QL, Wang H (2007) Study on accumulation of Zn in Stropharia rugoso-annulate in liquid culture. Agric Technol 27:58–59

    Google Scholar 

  42. Zhao SJ, Shi GA, Dong XC (2002) Experiment instruction of plant physiology. China Agricultural Technology Press, Beijing

    Google Scholar 

  43. Zhong M, Gao D, Hu K, Chen LJ, Zhang L, Li HG, Zhang Y (2009) The Effect of selenium-enrich Cordyceps militaris on hypolipidemic and anti-oxidant on mice. Li Shizhen Med Mat Med Res 20:3143–3144

    Google Scholar 

  44. Zou X, Zhang KC (2003) Effect of enriched Zn culture on growth and metabolism of Agarieus blazei Murill. J Wuxi Univ Light Ind 22:8–12

    Google Scholar 

Download references

Acknowledgments

This study was supported by grants from Mushroom Industrial System Project of Shandong.

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Authors and Affiliations

  1. College of Life Science, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China

    Lan Zheng, Long Hao, Xinyi Sun & Le Jia

  2. The Central Hospital of Taian, Shandong, 271000, People’s Republic of China

    Hua Ma

  3. Taian Health Inspection Institute, Taian, Shandong, 271000, People’s Republic of China

    Chengye Tian

  4. College of Mathematics and Applied Mathematics, Fudan University, Shanghai, 200433, People’s Republic of China

    Tong Li

  5. The Second High School of Taian, Shandong, 271000, People’s Republic of China

    Mengshi Jia

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  1. Lan Zheng
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  2. Long Hao
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  3. Hua Ma
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  4. Chengye Tian
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  5. Tong Li
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  8. Le Jia
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Correspondence to Le Jia.

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Lan Zheng, Hua Ma and Chengye Tian, contributed equally to this work.

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Zheng, L., Hao, L., Ma, H. et al. Production and in Vivo Antioxidant Activity of Zn, Ge, Se-enriched Mycelia by Cordyceps sinensis SU-01. Curr Microbiol 69, 270–276 (2014). https://doi.org/10.1007/s00284-014-0582-z

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  • DOI: https://doi.org/10.1007/s00284-014-0582-z

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