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Characteristics of Se-Enriched Mycelia by Stropharia rugoso-annulata and its Antioxidant Activities in vivo

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Abstract

Selenium (Se) is an essential trace element for humans and animals. Stropharia rugoso-annulata is a nutritional and functional mushroom containing many kinds of bioactive ingredients. The aims of this study were to investigate the Se-enrichment characteristics of S. rugoso-annulata in submerged culture and evaluate the antioxidant activities of Se-enriched mycelia in vivo in terms of the values of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and malondialdehyde (MDA). The optimum parameters of Se-enrichment under the optimal Se concentration (150 μg/mL) in media were as follows: biomass 8.11 ± 0.25 g/L, Se content in mycelia 4,727.68 ± 13 μg/g, Se-accumulated rate 24.68 ± 1.67%, and percentage of organic Se 96.27 ± 3.26%. The mainly subsistent forms of selenium in Se-enriched mycelia were selenoprotein and selenium-polysaccharide. The contents of total amino acids (TAA) and essential amino acids (EAA) in Se-enriched mycelia were increased by 13.5 ± 1.09% and 12.8 ± 0.89%, respectively. It was efficient for Se-enriched mycelia to elevate GSH-Px and SOD activities and decrease MDA content. These results indicated that Se-enriched mycelia of S. rugoso-annulata represent a novel dietary source of bioavailable supplemental selenium.

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Acknowledgements

The authors gratefully acknowledge the financial supports by the Natural Science Fund Program of Shandong (Y2006D08) and Doctor Fund Program of Shandong (23466).

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  1. College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China

    Zhen Song, Le Jia, Feng Xu, Fanyun Meng, Peng Deng, Keming Fan & Xiaonan Liu

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  1. Zhen Song
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  2. Le Jia
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  3. Feng Xu
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  4. Fanyun Meng
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Correspondence to Le Jia.

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Song, Z., Jia, L., Xu, F. et al. Characteristics of Se-Enriched Mycelia by Stropharia rugoso-annulata and its Antioxidant Activities in vivo. Biol Trace Elem Res 131, 81–89 (2009). https://doi.org/10.1007/s12011-009-8343-8

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  • DOI: https://doi.org/10.1007/s12011-009-8343-8

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