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A Staining Method for Assessing the Viability of Esteya vermicola Conidia

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Abstract

The viability of conidia of Esteya vermicola, a potentially important biocontrol agent against the pinewood nematode Bursaphelenchus xylophilus, is usually determined by cultivation for 18–48 h in culture medium. As an alternative to this labor-intensive method, we have developed a rapid, simple, and low-cost staining method for assessing E vermicola conidia survival rates. A mixture of neutral red and methylene blue was found to be the most optimal among several stains that also included safranin O and Janus green B. This mixture stained nonviable conidia blue, in contrast to viable conidia, which were stained red in the cytoplasm and blue in the cell wall. This method may be particularly useful for traditional research laboratories, as it provides rapid results using common, relatively inexpensive laboratory equipment.

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Authors and Affiliations

  1. Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon, 305-764, South Korea

    Yunbo Wang, NguyenTrong Thang, Zheng Li, Jingjie Li, Jianjie Xue, Lijuan Gu, VuThuy Hong, Lee Mira & Changkeun Sung

  2. Chinese Academy of Forestry, Beijing, 100091, People’s Republic of China

    Yongan Zhang

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  1. Yunbo Wang
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  2. NguyenTrong Thang
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  3. Zheng Li
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  4. Yongan Zhang
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  5. Jingjie Li
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  6. Jianjie Xue
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  7. Lijuan Gu
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  8. VuThuy Hong
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  9. Lee Mira
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  10. Changkeun Sung
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Correspondence to Changkeun Sung.

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Wang, Y., Thang, N., Li, Z. et al. A Staining Method for Assessing the Viability of Esteya vermicola Conidia. Curr Microbiol 69, 53–55 (2014). https://doi.org/10.1007/s00284-014-0553-4

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  • DOI: https://doi.org/10.1007/s00284-014-0553-4

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