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Expression of CspE by a Psychrotrophic Bacterium Enterobacter ludwigii PAS1, Isolated from Indian Himalayan Soil and In silico Protein Modelling, Prediction of Conserved Residues and Active Sites

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Abstract

Proteome analysis of Enterobacter ludwigii PAS1 provide a powerful set of tool to study the cold shock proteins along with that combination of bioinformatics is useful for interpretation of comparative results from many species. There is a considerable interest in the use of psychrotrophic bacteria for nitrogen fixation, especially at hilly regions, thus better understanding of cold adaptation mechanisms too. The psychrotrophic E. ludwigii PAS1 grown at 30 and 4 °C, isolated from Himalaya soil was undertaken for proteomic responses during optimal and cold shock conditions. Comparative proteomic analyses using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF/TOF MS revealed the presence of Cold shock protein E (CspE). Three-dimensional structure of CspE of E. ludwigii PAS1 divulge the presence of five antiparallel β-sheets forming a β-barrel structure with surface exposed aromatic and basic residues that were responsible for nucleic acid binding and also reveals the presence of highly conserved nucleic acid-binding motifs RNP1 and RNP2 in Csp family.

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Acknowledgments

This work is supported by National Bureau of Agriculturally Important Microorganisms (NBAIM)/Indian Council of Agricultural Research (ICAR) Grant to RG. We also acknowledge Director, CSIR-CIMAP, Lucknow for providing the lab facilities.

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Authors and Affiliations

  1. Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants, (Council of Scientific and Industrial Research), Lucknow, 226015, Uttar Pradesh, India

    Premalatha Kandasamy, Nidarshana Chaturvedi, Brijesh S. Sisodia & Ajit K. Shasany

  2. Bioinformatics Lab, National Bureau of Plant Genetic Resources, New Delhi, India

    Shachi Gahoi & Soma S. Marla

  3. Department of Microbiology, College of Basic Sciences and Humanities, G.B.Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, India

    Reeta Goel

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  1. Premalatha Kandasamy
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  2. Nidarshana Chaturvedi
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Correspondence to Premalatha Kandasamy.

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Kandasamy, P., Chaturvedi, N., Sisodia, B.S. et al. Expression of CspE by a Psychrotrophic Bacterium Enterobacter ludwigii PAS1, Isolated from Indian Himalayan Soil and In silico Protein Modelling, Prediction of Conserved Residues and Active Sites. Curr Microbiol 66, 507–514 (2013). https://doi.org/10.1007/s00284-013-0304-y

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  • DOI: https://doi.org/10.1007/s00284-013-0304-y

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