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Geographic and Tissue Influences on Endophytic Fungal Communities of Taxus chinensis var. mairei in China

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Abstract

Endophytc fungi were collected from the barks, branches and leaves of Taxus chinensis var. mairei from the Jiangxi, Zhejiang and Chongqing regions of China and their influences on geographic and tissue investigated. A total of 145 fungal taxa were identified based on molecular techniques, of these 125 taxa (86.2 %) belonging to Ascomycota, 14 (9.7 %) to Basidiomycota, 5 (3.4 %) to Zygomycota, and 1 (0.7 %) to undefined fungi. The species richness and diversity of endophytic fungi were significantly affected by tissue, and were 1.2–2.5-fold higher in the branches and barks when compared to the leaves. The locality affected the species richness per tree and the shannon diversity index per tree by longitude. The endophyte assemblages were strongly shaped by locality and tissue according to partial least squares discriminant analysis. In addition, the distributions of dominant fungi at orders and genera levels differed as a function of locality and tissue. Most of the dominant taxa showed spatial heterogeneity and tissue specificity or preference and many fungal taxa with low frequency were special to one locality or one tissue.

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Acknowledgments

We thank Chengjian Zheng, Ph.D. (Second Military Medical University, Shanghai), Professor Jinping Si (Zhejiang A & F University, Zhejiang), Xianbao Deng (Jinyun Mountain Natural Reservation, Chongqing) for help in collecting T. chinensis var. mairei plant samples.

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Authors and Affiliations

  1. Lab of Endophytic Fungi, Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325# Guohe Road, Shanghai, People’s Republic of China

    Lingshang Wu, Ting Han, Wenchao Li, Min Jia, Liming Xue & Luping Qin

  2. Research & Development Center of Natural Medicine, School of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, Lin’an, Hangzhou, Zhejiang, People’s Republic of China

    Lingshang Wu

  3. Faculty of Science, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK

    Khalid Rahman

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  1. Lingshang Wu
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Correspondence to Ting Han or Luping Qin.

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Wu, L., Han, T., Li, W. et al. Geographic and Tissue Influences on Endophytic Fungal Communities of Taxus chinensis var. mairei in China. Curr Microbiol 66, 40–48 (2013). https://doi.org/10.1007/s00284-012-0235-z

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