Abstract
Transcriptional regulation of genes encoding chitin synthases (CHS) and β-1,3-glucan synthase (GLS) from Ustilago maydis was studied. Transcript levels were measured during the growth curve of yeast and mycelial forms, in response to ionic and osmotic stress, and during infection of maize plants. Expression of the single GLS gene was constitutive. In contrast, CHS genes expression showed differences depending on environmental conditions. Transcript levels were slightly higher in the mycelial forms, the highest levels occurring at the log phase. Ionic and osmotic stress induced alterations in the expression of CHS genes, but not following a defined pattern, some genes were induced and others repressed by the tested compounds. Changes in transcripts were more apparent during the pathogenic process. At early infection stages, only CHS6 gene showed significant transcript levels, whereas at the period of tumor formation CHS7 and CHS8 genes were also were induced.
Similar content being viewed by others
References
Banuett F, Herskowitz I (1989) Different a alleles of Ustilago maydis are necessary for maintenance of filamentous growth but not for meiosis. Proc Natl Acad Sci USA 86:5878–5882
Banuett F, Herskowitz I (1994) Morphological transitions in the life cycle of Ustilago maydis and their genetic control by the a and b loci. Exp Mycol 18:247–266
Cervantes-Chávez JA, Ortiz-Castellanos L, Tejeda-Sartorius M, Gold S, Ruiz-Herrera J (2010) Functional analysis of the pH responsive pathway Pal/Rim in the phytopathogenic basidiomycete Ustilago maydis. Fungal Genet Biol 47:446–457
Chen-Wu JL, Zwicker J, Bowen AR, Robbins PW (1992) Expression of chitin synthase genes during yeast and hyphal growth phases of Candida albicans. Mol Microbiol 6:497–502
Feldbrügge M, Kämper J, Steinberg G, Kahmann R (2004) Regulation of mating and pathogenic development in Ustilago maydis. Curr Opin Microbiol 7:666–672
Garcerá-Teruel A, Xoconostle-Cázares B, Rosas-Quijano R, Ortiz L, León-Ramírez C, Specht CA, Sentandreu R, Ruiz-Herrera J (2004) Loss of virulence in Ustilago maydis by Umchs6 gene disruption. Res Microbiol 155:87–97
Gold SE, Kronstad JW (1994) Disruption of two genes for chitin synthase in the phytopathogenic fungus Ustilago maydis. Mol Microbiol 11:897–902
Holliday R (1961) The genetics of Ustilago maydis. Genet Res 2:204–230
Huang Z, Fasco MJ, Kaminsky LS (1996) Optimization of DNAse I removal of contaminating DNA from RNA for use in quantitative RNA-PCR. Biotechniques 20:1012–1020
Klose J, de Sá MM, Kronstad JW (2004) Lipid-induced filamentous growth in Ustilago maydis. Mol Microbiol 52(823):835
Klosterman SJ, Perlin MH, Garcia-Pedrajas M, Covert SF, Gold SE (2007) Genetics of morphogenesis and pathogenic development of Ustilago maydis. Adv Genet 57:1–47
Liu H, Kauffman S, Becker JM, Szaniszlo PJ (2004) Wangiella (Exophiala) dermatitidis WdChs5p, a class V chitin synthase, is essential for sustained cell growth at temperature of infection. Eukaryot Cell 3:40–51
López-Matas MA, Eslava AP, Diaz-Minguez JM (2000) Mcchs1, a member of a chitin synthase gene family in Mucor circinelloides, is differentially expressed during dimorphism. Curr Microbiol 40:169–175
Martinez-Espinoza AD, León C, Elizarraraz G, Ruiz-Herrera J (1997) Monomorphic non-pathogenic mutants of Ustilago maydis. Phytopathology 87:259–265
Mazur P, Morin N, Baginsky W, el-Sherbeini M, Clemas JA, Nielsen JB, Foor F (1995) Differential expression and function of two homologous subunits of yeast 1,3-β-d-glucan synthase. Mol Cell Biol 15:5671–5681
Merz RA, Horsch M, Nyhlén LE, Rast D (1999) Biochemistry of chitin synthase. In: Jolles P, Muzzarelli RAA (eds) Chitin and chitinases. Birkhauser Verlag, Basel, pp 9–37
Niño-Vega GA, Munro CA, San-Blas G, Gooday GW, Gow NA (2000) Differential expression of chitin synthase genes during temperature-induced dimorphic transitions in Paracoccidioides brasiliensis. Med Mycol 38:31–39
Reverberi M, Di Mario F, Tomati U (2004) β-Glucan synthase induction in mushrooms grown on olive mill wastewaters. Appl Microbiol Biotechnol 66:217–225
Ruiz-Herrera J, Elorza MV, Alvarez PE, Sentandreu R (2004) Biosynthesis of the fungal cell wall. In: San-Blas G, Calderone NA (eds) Pathogenic fungi, structural: biology and taxonomy. Caister Academic Press, Wymondham, pp 41–99
Ruiz-Herrera J, González-Prieto JM, Ruiz-Medrano R (2001) Evolution and phylogenetic relationships of chitin synthases from yeasts and fungi. FEMS Yeast Res 1:247–256
Ruiz-Herrera J, León CG, Guevara-Olvera L, Carabez-Trejo A (1995) Yeast-mycelial dimorphism of haploid and diploid strains of Ustilago maydis. Microbiology 141:695–770
Ruiz-Herrera J, Martínez-Espinoza AD (1998) The fungus Ustilago maydis, from the aztec cuisine to the research laboratory. Int Microbiol 1:149–158
San-Blas G, Niño-Vega G, Iturriaga T (2002) Paracoccidioides brasiliensis and paracoccidioidomycosis: molecular approaches to morphogenesis, diagnosis, epidemiology, taxonomy, and genetics. Med Mycol 40:225–242
Sentandreu R, Elorza MV, Valentín E, Ruiz-Herrera J (2004) The structure and composition of fungal cell wall. In: San-Blas G, Calderone NA (eds) Pathogenic fungi: structural, biology, and taxonomy. Caister Academic Press, Wymondham, pp 3–27
Tartar A, Shapiro AM, Scharf DW, Boucias DG (2005) Differential expression of chitin synthase (CHS) and glucan synthase (FKS) genes correlates with the formation of a modified, thinner cell wall in in vivo produced Beauveria bassiana cells. Mycopathologia 160:303–314
Vollmeister E, Schipper K, Baumann S, Haag C, Pohlmann T, Stock J, Feldbrügge M (2012). Fungal development of the plant pathogen Ustilago maydis. FEMS Microbiol Rev 36:59–77
Xoconostle-Cazares B, Leon-Ramirez C, Ruiz-Herrera J (1996) Two chitin synthase genes from Ustilago maydis. Microbiology 142:377–387
Xoconostle-Cázares B, Specht CA, Robbins PW, Liu Y, León C, Ruiz-Herrera J (1997) Umchs5, a gene coding for a class IV chitin synthase in Ustilago maydis. Fungal Genet Biol 22:199–208
Weber I, Assmann D, Thines E, Steinberg G (2006) Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis. Plant Cell 18:225–242
Acknowledgments
This study was partially supported by Consejo Nacional de Ciencia y Tecnología (CONACYT), México. MRB is a D. Sc. student supported by a fellowship from CONACYT. Thanks are given to Claudia León-Ramírez for her help during this investigation.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Robledo-Briones, M., Ruiz-Herrera, J. Transcriptional Regulation of the Genes Encoding Chitin and β-1,3-Glucan Synthases from Ustilago maydis . Curr Microbiol 65, 85–90 (2012). https://doi.org/10.1007/s00284-012-0129-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00284-012-0129-0