Skip to main content
SpringerLink
Log in

Activity and expression of laccase, tyrosinase, glucanase, and chitinase genes during morphogenesis of Lentinus edodes

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

Activation of expression of the lcc4 and tir genes encoding laccase and tyrosinase was observed during transition of the xylotrophic basidiomycete Lentinus edodes from the vegetative to the generative growth stages. This was especially pronounced in the brown mycelial mat (the stage preceding formation of the fruiting bodies). Development of this structure was shown to be associated with a sharp increase in laccase and tyrosinase activities, as well as with rearrangements in the phenol oxidase complex. Formation of the tissues with thickened cell walls was associated with enhanced expression of the chi and exg1 genes encoding chitinase and glucanase, respectively. Exogenous treatment of the vegetative mycelium with a laccase preparation from the brown mycelial mat promoted formation of this morphological structure. Activation of the lcc4, tir, chi, and exg1 genes may be used as a marker of readiness to fruition in xylotrophic fungi.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Feofilova, E.P., Mycelial fungi as a source for obtaining new medical products with immunomodulating, antitumoral, and wound healing activities, Immunopathol., Allergol., Infectol. (Moscow), 2004, vol. 1, pp. 27–33.

    Google Scholar 

  2. Bender, S., Lonergan, G.T., Backhaus, J., Cross, R.F., and Baker, W.L., The antibiotic activity of the edible and medicinal mushroom Lentinus edodes (Berk.) Sing., Int. J. Medicinal Mushrooms, 2001, vol. 3, nos. 2–3, p. 118.

    Google Scholar 

  3. Okeke, B.C., Paterson, A., Smith, J.E., and WatsonCraik, I.A., The relationship between phenoloxidase activity, soluble protein and ergosterol with growth of Lentinus species in oak sawdust logs, Appl. Microbiol. Biotechnol., 1994, vol. 41, pp. 28–31.

    Article  CAS  Google Scholar 

  4. Thurston, C.F., The structure and function fungal laccase, J. Microbiol., 1994, vol. 140, pp. 19–26.

    Article  CAS  Google Scholar 

  5. Leatham, G.F. and Stahmann, M.A., Studies on the laccase of Lentinus edodes: specificity, localization and association with the development of fruiting bodies, J. Gen. Microbiol., 1981, vol. 125, pp. 147–157.

    CAS  Google Scholar 

  6. Das, N., Sengupta, S., and Mukherjee, M., Importance of laccase in vegetative growth of Pleurotus florida, Appl. Environ. Microbiol., 1997, vol. 63, pp. 4120–4122.

    CAS  PubMed Central  PubMed  Google Scholar 

  7. Wood, D.A., Production, purification and properties of extracellular laccase of Agaricus bisporus, J. Gen. Microbiol., 1980, vol. 117, pp. 327–338.

    CAS  Google Scholar 

  8. Van Gelder, C., Flurkey, W., and Wichers, H., Sequence and structural features of plant and fungal tyrosinases, Phytochemistry, 1997, vol. 45, pp. 1309–1323.

    Article  PubMed  Google Scholar 

  9. Cabib, E., Bowers, B., Sburlati, A., and Silverman, S.J., Fungal cell wall synthesis: the construction of a biological structure, Microbiol. Sci., 1988, vol. 5, pp. 370–375.

    CAS  PubMed  Google Scholar 

  10. Fontaine, T., Hartland, R.P., Beauvais, A., Diaquin, M., and Latge, J.P., Purification and characterization of an endo-l,3-β-glucanase from Aspergillus fumigatus, Eur. J. Biochem., 1997, vol. 243, pp. 315–321.

    Article  CAS  PubMed  Google Scholar 

  11. Feofilova, E.P., Key role of chitin in fungal cell wall, in Khitin i khitozan: poluchenie, svoistva i primenenie (Chitin and Chitosan: Production, Properties, and Application), Moscow: Nauka, 2002, pp. 91–111.

    Google Scholar 

  12. Feofilova, E.P., Kletochnaya stenka gribov (Fungal Cell Wall), Moscow: Nauka, 1983.

    Google Scholar 

  13. Vetchinkina, E.P. and Nikitina, V.E., Morphological patterns of mycelial growth and fruition of some strains of an edible xylotrophic basidiomycete Lentinus edodes, Izv. Samar. Nauch. Tsentr. Ross. Akad. Sci., 2007, vol. 9, no. 4, pp. 1085–1090.

    Google Scholar 

  14. Vetchinkina, E.P. and Nikitina, V.E., Comparative analysis of the proteinaceous complex in the morphological structures of a culturable xylotrophic basidiomycete Lentinus edodes, Mikol. Fitopatol., 2008, vol. 42, no. 2, pp. 173–177.

    CAS  Google Scholar 

  15. Osterman, L.A., Metody issledovaniya belkov i nukleinovykh kislot: Elektroforez i ul’tratsentrifugirovanie, (Methods for Investigation of Proteins and Nucleic Acids: Electrophoresis and Ultracentrifugation), Moscow: Nauka, 1981.

    Google Scholar 

  16. Sakamoto, Yu., Nakade, K., and Sato, T., Characterization of the post-harvest changes in gene transcription in the gill of the Lentinula edodes fruiting body, Curr. Genet., 2009, vol. 55, pp. 409–423.

    Article  CAS  PubMed  Google Scholar 

  17. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, no. 5259, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  18. Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, vol. 72, pp. 248–258.

    Article  CAS  PubMed  Google Scholar 

  19. Gaal’, E., Med’eshi, G., and Veretski, L., Elektroforez v razdelenii biologicheskikh makromolekul (Electrophoresis in the Separation of Biological Macromolecules), Moscow: Mir, 1982.

    Google Scholar 

  20. Pomerantz, S.H. and Murthy, V.V., Purification and properties of tyrosinases from Vibrio tyrosinaticus, Arch. Biochem. Biophys., 1974, vol. 160, no. 1, pp. 73–82.

    Article  CAS  PubMed  Google Scholar 

  21. Slomczynski, D., Nakas, J.P., and Tanenbaum, S.W., Production and characterization of laccase from Botrytis cinerea 61-34, Appl. Environ. Microbiol., 1995, vol. 61, pp. 907–912.

    CAS  PubMed Central  PubMed  Google Scholar 

  22. Reynolds, E.S., The use of lead citrate at high pH as an electron opaque stain in electron microscopy, J. Cell Biol., 1963, vol. 17, no. 1, pp. 208–212.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. Whitaker, J.R., Polyphenol oxidase, in Food Enzymes: Structure and Mechanism, Wong, D.W.S., Ed., New York: Chapman and Hall, 1995, pp. 271–307.

    Chapter  Google Scholar 

  24. Herrera-Estrella, A. and Chet, I., Chitinases in biological control, in Chitin and Chitinases, Jolles, P. and Muzarelli, R., Eds., Basel: Birkhausen, 1999, pp. 171–184.

    Chapter  Google Scholar 

  25. Bartnicki-Garcia, S., Fundamental aspects of hyphal morphogenesis, Symp. Soc. Gen. Microbiol., 1973, no. 23, pp. 245–257.

    Google Scholar 

  26. Michalenko, G.O., Hohl, H.L., and Rast, D., Chemistry and architecture of the mycelial wall of Agaricus bisporus, J. Gen. Microbiol., 1976, vol. 92, pp. 252–262.

    Article  Google Scholar 

  27. Bowman, S.M. and Free, S.J., The structure and synthesis of the fungal cell wall, BioEssays, 2006, vol. 28, no. 8, pp. 799–808.

    Article  PubMed  Google Scholar 

  28. Kamada, T., Fujii, T., Nakagawa, T., and Takenaru, T., Changes in l,3-β-glucanase activities during stipe elongation in Coprinus cinereus, Curr. Microbiol., 1985, vol. 12, pp. 251–260.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia

    E. P. Vetchinkina & V. E. Nikitina

  2. Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan, Russia

    V. Y. Gorshkov, M. V. Ageeva & Y. V. Gogolev

Authors
  1. E. P. Vetchinkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Y. Gorshkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. V. Ageeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. V. Gogolev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. E. Nikitina
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. P. Vetchinkina.

Additional information

Original Russian Text © E.P. Vetchinkina, V.Y. Gorshkov, M.V. Ageeva, Y.V. Gogolev, V.E. Nikitina, 2015, published in Mikrobiologiya, 2015, Vol. 84, No. 1, pp. 78–89.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vetchinkina, E.P., Gorshkov, V.Y., Ageeva, M.V. et al. Activity and expression of laccase, tyrosinase, glucanase, and chitinase genes during morphogenesis of Lentinus edodes . Microbiology 84, 49–58 (2015). https://doi.org/10.1134/S0026261715010166

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261715010166

Keywords

Search

Navigation