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Two Types of Phytases (Histidine Acid Phytase and β-Propeller Phytase) in Serratia sp. TN49 from the Gut of Batocera horsfieldi (Coleoptera) Larvae

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Abstract

Microbial phytases play a major role in the mineralization of organic phosphorous, especially in symbiotic plants and animals. In this study, we identified two types of phytases in Serratia sp. TN49 that was harbored in the gut of Batocera horsfieldi (Coleoptera) larvae. The two phytases, an acidic histidine acid phosphatase (PhyH49) and an alkaline β-propeller phytase (PhyB49), shared low identities with known phytases (61% at most). PhyH49 and PhyB49 produced in Escherichia coli exhibited maximal activities at pH 5.0 (60°C) and pH 7.5–8.0 (45°C), respectively, and are complementary in phytate degradation over the pH range 2.0–9.0. Serratia sp. TN49 harboring both PhyH49 and PhyB49 might make it more adaptive to environment change, corresponding to the evolution trend of microorganism.

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Acknowledgment

This research was supported by the National Natural Science Foundation of China (31001025) and the China Modern Agriculture Research System (CARS-42).

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Authors and Affiliations

  1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China

    Rui Zhang, Peilong Yang, Huoqing Huang, Pengjun Shi, Tiezheng Yuan & Bin Yao

  2. College of Life Sciences, Yunnan Normal University, Kunming, China

    Rui Zhang

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  1. Rui Zhang
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  2. Peilong Yang
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  3. Huoqing Huang
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  4. Pengjun Shi
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  5. Tiezheng Yuan
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  6. Bin Yao
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Correspondence to Bin Yao.

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Zhang, R., Yang, P., Huang, H. et al. Two Types of Phytases (Histidine Acid Phytase and β-Propeller Phytase) in Serratia sp. TN49 from the Gut of Batocera horsfieldi (Coleoptera) Larvae. Curr Microbiol 63, 408 (2011). https://doi.org/10.1007/s00284-011-9995-0

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