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Microarray Analysis of the Chelerythrine-Induced Transcriptome of Mycobacterium tuberculosis

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Abstract

Chelerythrine (a natural quaternary benzophenanthridine alkaloid) is an extract from the roots of Chelidonium majus with potential antimycobacterial activity. To reveal the possible mechanism of action of chelerythrine against Mycobacterium tuberculosis (M. tuberculosis), commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes triggered by treatment with subinhibitory concentrations of chelerythrine. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data using Agilent software. Analysis of the microarray data revealed that a total of 759 genes were differentially regulated by chelerythrine. Of these, 372 genes were upregulated, and 387 genes were downregulated. Some of the important genes that were significantly regulated are related to different pathways (such as urease), methoxy-mycolic acid synthase, surface-exposed lipids, the heat shock response, and protein synthesis. This genome-wide transcriptomics approach produced the first insights into the response of M. tuberculosis to a chelerythrine challenge.

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Acknowledgments

This work was supported by Important National Science, Technology Specific Projects (2008ZX10301), the Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) (No. 200801831051), the National Nature Science Foundation of China (No. 30871889), the Fund for Science and Technology Development of the Jilin Province, China (No. 200705233), and the Fundamental Research Funds for the Central Universities.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China

    Junchao Liang, Lei Li, Jing Jin, Xiuping Wu, Mingyuan Liu, Yang Li & Lu Yu

  2. Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, 100730, China

    Qi Jin

  3. Department of Food Quality and Safety, College of Quartermaster Technology, Jilin University, Changchun, 130062, China

    Na Guo

  4. The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China

    Aizhen Guo & Liqiang Liu

  5. Department of Animal Science Technology, Jilin Agricultural University, Changchun, 130118, China

    Fanli Zeng & Dan Zhao

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  1. Junchao Liang
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  2. Fanli Zeng
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  3. Aizhen Guo
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  4. Liqiang Liu
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  10. Dan Zhao
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  11. Yang Li
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  12. Qi Jin
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  13. Lu Yu
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Corresponding authors

Correspondence to Qi Jin or Lu Yu.

Additional information

Junchao Liang, Fanli Zeng, and Aizhen Guo contributed equally to this work.

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Liang, J., Zeng, F., Guo, A. et al. Microarray Analysis of the Chelerythrine-Induced Transcriptome of Mycobacterium tuberculosis . Curr Microbiol 62, 1200–1208 (2011). https://doi.org/10.1007/s00284-010-9837-5

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  • DOI: https://doi.org/10.1007/s00284-010-9837-5

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