Abstract
Association between cold stress and oxidative stress was demonstrated by measuring the activity of two antioxidant enzymes and the level of free radicals generated in two batches of cells of an Antarctic bacterium Pseudomonas fluorescens MTCC 667, grown at 22 and 4°C. Increase in oxidative stress in cells grown at low temperature was evidenced by increase in the activity of an enzyme and also in the amount of free radicals generated, in the cold-grown cells. The association between cold stress and oxidative stress demonstrated in this investigation bolsters the concept of interlinked stress response in bacteria.
Similar content being viewed by others
References
Chattopadhyay MK (2006) Mechanism of bacterial adaptation to low temperature. J Biosci 31:157–165
Chattopadhyay MK (2002) Low temperature and oxidative stress. Curr Sci 83:109
Smirnova GV, Zakirova ON, Oktiabr’skii ON (2001) Role of the antioxidant system in response of Escherichia coli bacteria to cold stress. Mikrobiologiia 70:55–60
Liu S, Graham JE, Bigelow L, Morse PD II, Wilkinson BJ (2002) Identification of Listeria monocytogenes genes expressed in response to growth at low temperature. Appl Environ Microbiol 68:1697–1705
Hossain MM, Nakamoto H (2003) Role for the cyanobacterial HtpG in protection from oxidative stress. Curr Microbiol 46:70–76
Gocheva YG, Tosi S, Krumova ET, Slokoska LS, Miteva JG, Vassilev Sv, Angelova MB (2009) Temperature downshift induces antioxidant response in fungi isolated from Antarctica. Extremophiles 13:273–281
Shivaji S, Rao NS, Saisree L, Sheth V, Reddy GSN, Bhargava PM (1989) Isolation and identification of Pseudomonas spp. from Schirmacher Oasis, Antarctica. Appl Environ Microbiol 55:767–770
Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
Samuni A, Carmichael AJ, Russo A, Mitchell J, Riesz P (1986) On the spin trapping and ESR detection of oxygen-derived radicals generated inside cells. Proc Natl Acad Sci USA 83:7593–7597
Chaudière J, Ferrari-Iliou R (1999) Intracellular antioxidants: from chemical to biochemical mechanisms. Food Chem Toxicol 37:949–962
Regha K, Satapathy AK, Ray MK (2005) RecD plays an essential function during growth at low temperature in the Antarctic bacterium Pseudomonas syringae Lz4W. Genetics 170:1473–1484
Pavankumar TL, Sinha AK, Ray MK (2010) All three subunits of Rec BCD enzyme are essential for DNA repair and low-temperature growth in the Antarctic Pseudomonas syringae LZ 4W. PloS One 5:e9412
Chattopadhyay MK (2008) Cryotolerance in bacteria: Interlink with adaptation to other stress factors. Trends Microbiol 16:455
Acknowledgments
The investigators are thankful to the Council of Scientific and Industrial Research, New Delhi, India and University Grants Commission, New Delhi, India for providing funds for this investigation. The technical help provided by Mr C. Suresh, University of Hyderabad, India in recording the EPR spectra, is also sincerely acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chattopadhyay, M.K., Raghu, G., Sharma, Y.V.R.K. et al. Increase in Oxidative Stress at Low Temperature in an Antarctic Bacterium. Curr Microbiol 62, 544–546 (2011). https://doi.org/10.1007/s00284-010-9742-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00284-010-9742-y