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Promotion of Monacolin K Production by Agrobacterium tumefaciens-Mediated Transformation in Monascus albidus 9901

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Abstract

The binary vector pCAMBIA3300-gpdA-hph-trpC with hygromycin B phosphotransferase (hph) was constructed and transformed into Monascus albidus 9901 by Agrobacterium tumefaciens-mediated transformation, with gene hph as the selective marker. In order to improve the efficiency of A. tumefaciens-mediated transformation in M. albidus 9901, we optimized various factors including concentration of M. albidus 9901 spores, cell density of A. tumefaciens, co-cultivation time, temperature, and acetosyringone concentration. Most transformants of M. albidus 9901 could grow stably on media containing 50 μg ml−1 hygromycin B up to five generations. The presence of hph was identified by PCR. Two transformants H1 and H2 which produced more Monacolin K than M. albidus 9901 were screened, and the concentration of Monacolin K in the fermented millet by H1 and H2 increased by 42.15% and 40.34% respectively compared with that produced by M. albidus 9901.

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Acknowledgment

This study was supported by National Key Technology R&D Program in the 11th Five year Plan of China (No. 2006BAD27B09-3).

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  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu Province, China

    Lu Wang, Wu Wang & Ganrong Xu

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  1. Lu Wang
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  2. Wu Wang
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  3. Ganrong Xu
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Correspondence to Lu Wang.

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Wang, L., Wang, W. & Xu, G. Promotion of Monacolin K Production by Agrobacterium tumefaciens-Mediated Transformation in Monascus albidus 9901. Curr Microbiol 62, 501–507 (2011). https://doi.org/10.1007/s00284-010-9735-x

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  • DOI: https://doi.org/10.1007/s00284-010-9735-x

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