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Growth, Fe3+ Reductase Activity, and Siderophore Production by Paenibacillus polymyxa SQR-21 Under Differential Iron Conditions

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Abstract

An experiment was planned to evaluate the behavior of Paenibacillus polymyxa SQR-21 under differential iron availability. P. polymyxa was grown under three differential iron conditions (0, 2, 20 μM). Iron starvation slowed bacterial growth and at all iron levels, pH of liquid culture was decreased, but maximum decrease was observed at highest iron level. Cell surface ferrireductase activity decreased as culture aged, while extracellular Fe3+-reducing activity constantly increased. Hydroxamates type siderophores production was increased with the decrease in iron levels. Numerous cellular proteins were expressed by P. polymyxa in the range of 5–140 kDa and several of them showed conspicuous differential iron regulation. P. polymyxa seems to have more than one type of iron acquisition mechanism including gradual release of organic acids, cell surface ferrireductases, extracellular reductants, and secretion of low molecular weight hydroxamates chelators. This article is the first to report the kinetic study of P. polymyxa under differential iron availability. The information provided here gives initial information about the iron uptake mechanism of P. polymyxa.

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References

  1. Arnow LE (1937) Colorimetric determination of the components of 3,4-dihydroxyphenylalanine-tyrosine mixtures. J Biol Chem 18:531–537

    Google Scholar 

  2. Bhaduri S, Demchick PH (1983) Simple and rapid method for disruption of bacteria for protein studies. Appl Environ Microbiol 46:941–943

    CAS  PubMed  Google Scholar 

  3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  4. Csaky TZ (1948) On the estimation of bound hydroxylamine in biological materials. Acta Chem Scand 2:450–454

    Article  CAS  Google Scholar 

  5. Garner BL, Arceneaux JEL, Byers BR (2004) Temperature control of a 3,4-dihydroxybenzoate (protocatechuate)-based siderophore in Bacillus anthracis. Curr Microbiol 49:89–94

    CAS  PubMed  Google Scholar 

  6. Helbig J (2001) Biological control of Botrytis cinerea Pers. ex Fr. in strawberry by Paenibacillus polymyxa (Isolate 18191). J Phytopathol 149(5):265–273

    Article  Google Scholar 

  7. Homuth M, Peter VW, Rohde M, Gerlach GF (1998) Identification and characterization of a novel extracellular ferric reductase from Mycobacterium paratuberculosis. Infect Immun 66:710–716

    CAS  PubMed  Google Scholar 

  8. Lopez-Goni I, Moriyon I, Neilands JB (1992) Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore. Infect Immun 60:4496–4503

    CAS  PubMed  Google Scholar 

  9. de Luca NG, Wood PM (2000) Iron uptake by fungi: contrasted mechanisms with internal or external reduction. In: Poople RK (ed) Advances in microbial physiology. Sheffield, UK, pp 39–74

    Google Scholar 

  10. Machuca A, Milagres AMF (2003) Use of CAS-agar plate modified to study the effect of different variables on the siderophore production by Aspergillus. Lett Appl Microbiol 36:177–181

    Article  CAS  PubMed  Google Scholar 

  11. Mahmood M (1970) Trace elements for growth and bulbiformin production by Bacillus subtilis. J Appl Microbiol 35:1–5

    Article  Google Scholar 

  12. Morrissey JA, Williams PH, Cashmore AM (1996) Candida albicans has a cell-associated ferric-reductase activity, which is regulated in response to levels of iron and copper. Microbiology 142:485–492

    Article  CAS  PubMed  Google Scholar 

  13. Neilands JB (1984) Methodology of siderophores. Struct Bond 58:1–24

    Article  CAS  Google Scholar 

  14. Nyhus KJ, Wilborn AT, Jacobson ES (1997) Ferric iron reduction by Cryptococcus neoformans. Infect Immun 65:434–438

    CAS  PubMed  Google Scholar 

  15. Renshaw JC, Robson GD, Trinci APJ, Wiebe MG, Livens FR, Collison D, Taylor RJ (2002) Review: fungal siderophores—structures, functions and applications. Mycol Res 106:1123–1142

    Article  CAS  Google Scholar 

  16. Sharma PK, Rao KH (2001) Role of a heterotrophic Paenibacillus polymyxa bacteria in the bioflotation of some sulfide minerals, p. 67. In: Kawatra SK, Natarajan KA (eds) Mineral biotechnology. Society for Mining, Metallurgy and Exploration, Littleton

    Google Scholar 

  17. Siegel LS (1981) An International journal effect of iron on growth and toxin production by Clostridium botulinum Type A. Curr Microbiol 6:127–130

    Article  CAS  Google Scholar 

  18. Siddiqui ZA, Baghel G, Akhtar MS (2007) Biocontrol of Meloidogyne javanica by rhizobium and plant growth-promoting rhizobacteria on lentil. World J Microbiol Biotechnol 23:435–441

    Article  CAS  Google Scholar 

  19. Stookey LL (1970) Ferrozine: a new spectrophotometric reagent for iron. Anal Chem 42:779–781

    Article  CAS  Google Scholar 

  20. Temirov YV, Esikova TZ, Kashparov IA, Balashova TA, Vinokurov LM, Alakhov YB (2003) Catechol siderophore, produced by thermo-resistant strain of Bacillus licheniformis VK21. Russ J Bioorg Chem 29:542–549

    Article  CAS  Google Scholar 

  21. Timmerman MM, Woods JP (1999) Ferric reduction is a potential iron acquisition mechanism for Histoplasma capsulatum. Infect Immun 67:6403–6408

    CAS  PubMed  Google Scholar 

  22. Vartanian MD (1988) Differences in excretion and efficiency of the aerobactin and enterochelin siderophores in a bovine pathogenic strain of Escherichia coli. Infect Immun 56:413–418

    Google Scholar 

  23. Truper HG (2005) The type species of the genus Paenibacillus Ash et al. 1994 is Paenibacillus polymyxa. Opinion 77 Judicial Commission of the International Committee on Systematics of Prokaryotes Correspondence. Inter J Syst Evol Microbiol 55:513

    Article  Google Scholar 

  24. Zhang S, Raza W, Yang X, Hu J, Huang Q, Xu Y, Liu X, Shen Q (2008) Control of Fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer. Biol Fertil Soils 14(8):1073–1080

    Article  Google Scholar 

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Acknowledgments

This study was financially supported by National Nature Science Foundation of China (40871126) and China Science and Technology Ministry, 973 Program (2007CB109304) and 863 program (2006AAD10Z416), and China Agriculture Ministry (2006-G62). We thank Professor Warren Dick at Ohio State University, USA for his polishing the English of this article.

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Organic Waste Utilization, Nanjing Agricultural University, Nanjing, Jiangsu Province, People’s Republic of China

    Waseem Raza & Qirong Shen

  2. College of Resource and Environmental Sciences, Nanjing Agricultural University, No. 1, Weigang Road, Nanjing, 210095, Jiangsu Province, People’s Republic of China

    Qirong Shen

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  1. Waseem Raza
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  2. Qirong Shen
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Correspondence to Qirong Shen.

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Raza, W., Shen, Q. Growth, Fe3+ Reductase Activity, and Siderophore Production by Paenibacillus polymyxa SQR-21 Under Differential Iron Conditions. Curr Microbiol 61, 390–395 (2010). https://doi.org/10.1007/s00284-010-9624-3

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  • DOI: https://doi.org/10.1007/s00284-010-9624-3

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