Abstract
Biocontrol of the root-knot nematode Meloidogyne javanica was studied on lentil using plant growth-promoting rhizobacteria (PGPR) namely Pseudomonas putida, P. alcaligenes, Paenibacillus polymyxa and Bacillus pumilus and root nodule bacterium Rhizobium sp. Pseudomonas putida caused greater inhibitory effect on the hatching and penetration of M. javanica followed by P. alcaligenes, P. polymyxa and B. pumilus. Inoculation of any PGPR species alone or together with Rhizobium increased plant growth both in M. javanica-inoculated and -uninoculated plants. Inoculation of Rhizobum caused greater increase in plant growth than caused by any species of plant growth-promoting rhizobacteria in nematode-inoculated plants. Among PGPR, P. putida caused greater increase in plant growth and higher reduction in galling and nematode multiplication followed by P. alcaligenes, P. polymyxa and B. pumilus. Combined use of Rhizobium with any species of PGPR caused higher reduction in galling and nematode multiplication than their individual inoculation. Use of Rhizobium plus P. putida caused maximum reduction in galling and nematode multiplication followed by Rhizobium plus P. alcaligens. Pseudomonas putida caused greater root colonization and siderophore production followed by P. alcaligenes, P. polymyxa and B. pumilus. Analysis of the protein bands of these four species by SDS-PAGE revealed that P. putida had a different protein band profile compared to the protein profiles of P. alcaligenes, P. polymyxa and B. pumilus. However, the protein profiles of P. acaligenes, P. polymyxa and B. pumilus were similar.
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References
Aalten PM, Vitour D, Blanvillain D, Gowen SR, Sutra L (1998) Effect of rhizosphere fluorescent Pseudomonas strains on plant parasitic nematodes Radopholus similis and Meloidogyne spp. Lett Appl Microbiol 27:357–361
Ali SS (1997) Status of nematode problems and research in India. In: Diagnosis of key nematode pests of chickpea and pigeon pea in their management. Proceedings of Regional Training Course 25–30 Nov. 1996, ICRISAT, Patancheru, Andhra Pradesh, India, pp 74–82
Barker KR, Huisingh D (1970) Histopathological investigations of the antagonistic interaction between Heterodera glycines and Rhizobium japonicum on soybean. Phytopathology 60:1282–1283
Bora T, Ozaktan H, Gore E, Aslan E (2004) Biological control of Fusarium oxysporum f. sp. melonis by wettable powder formulations of two strains of Pseudomonas putida. J Phytopathol 152:471–475
Chakraborty U, Chakraborty BN (1989) Interaction of Rhizobium leguminosarum and Fusarium solani f.sp. pisi in pea affecting disease development and phytoalexin production. Can J Botany 67:1698–1701
Chakraborty U, Purkayastha RP (1984) Role of Rhizobitoxine in protecting soybean roots from Macrophomina phaseolina infection. Can J Microbiol 30:285–289
Dospekhov BA (1984) Field experimentation: statistical procedures. Mir publishers, Moscow, 352 pp (ISBN 6000133960)
Enebak SA, Carey WA (2000) Evidence for induced systemic protection to Fusarium rust in Loblolly pine by plant growth-promoting rhizosphere. Plant Disease 84:306–308
Gamliel A, Katan J (1993) Suppression of major and minor pathogens by fluorescent pseudomonads in solarized and nonsolarized soils. Phytopathology 83:68–75
Gokte N, Swarup G (1988) On the potential of some bacterial biocides against root-knot and cyst nematodes. Indian J Nematol 18:152–153
Haque SE, Gaffar A (1993) Use of rhizobia in the control of root-rot disease of sunflower, okra, soybean and mungbean. J Phytopathol 138:157–163
Kloepper JW, Leong J, Teintze M, Schroth MN (1980) Enhanced plant growth by plant growth-promoting rhizobacteria. Nature 286:885–886
Laemmli UK (1970) Cleavage of structural protein during the assembly of bacteriophage T4. Nature 227:680–685
Nelson LM (2004) Plant growth-promoting rhizobacteria (PGPR): prospects for new inoculants. Crop Management doi:101094/Cm-2004-0301-05-RV. See http://www.plantmanagementnetwork.org/pub/cm/review/2004/rhizobacteria/
Raupach GS, Kloepper JW (1998) Mixture of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology 88:1158–1164
Roslycky EB (1967) Bacteriocin production in the rhizobia bacteria. Can J Microbiol 13:431
Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56
Siddiqui ZA (2006) PGPR: prospective biocontrol agents of plant pathogens. In: Siddiqui ZA (ed) PGPR: biocontrol and biofertilization. Springer, The Netherlands, pp 111–142, 318 pp. ISBN 1402040024
Siddiqui ZA, Husain SI (1992) Interaction between Meloidogyne incognita race 3,Macrophomina phaseolina and Bradyrhizobium sp. in the root rot disease complex of chickpea, Cicer arietinum. Fund Appl Nematol 15:491–494
Siddiqui ZA, Mahmood I (1995) Role of plant symbionts in nematode management, a review. Bioresour Technol 54:217–226
Siddiqui ZA, Mahmood I (1999) Role of bacteria in the management of plant parasitic nematodes, a review. Bioresour Technol 69:167–179
Southey, JF (1986) Laboratory methods for work with plant and soil nematodes, 6th edn. Ministry of Agriculture Fisheries and Food. Reference book No. 402, HMSO, 202 pp. London, UK. ISBN 0112427545
Wei G, Kloepper JW, Tuzun S (1996) Induced systemic resistance to cucumber diseases and increased plant growth by plant growth-promoting rhizobacteria under field conditions. Phytopathology 86:221–224
Weller DM (1988) Biological control of soil borne plant-pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 26:379–407
Yan Z, Reddy MS, Ryu CM, McInroy JA, Wilson MA, Kloepper JW (2002) Induced systemic protection against tomato late blight elicited by plant growth-promoting rhizobacteria. Phytopathology 92:1329–1333
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The first author is grateful to D.S.T. New Delhi for the financial assistance through Project No. SP/SO/A22/2001 to carry out this work.
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Siddiqui, Z.A., Baghel, G. & Akhtar, M.S. Biocontrol of Meloidogyne javanica by Rhizobium and plant growth-promoting rhizobacteria on lentil. World J Microbiol Biotechnol 23, 435–441 (2007). https://doi.org/10.1007/s11274-006-9244-z
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DOI: https://doi.org/10.1007/s11274-006-9244-z