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New Insights in Trichoderma harzianum Antagonism of Fungal Plant Pathogens by Secreted Protein Analysis

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Abstract

Trichoderma harzianum ALL42 were capable of overgrowing and degrading Rhizoctonia solani and Macrophomina phaseolina mycelia, coiling around the hyphae with formation of apressoria and hook-like structures. Hyphae of T. harzianum ALL42 did not show any coiling around Fusarium sp. hyphae suggesting that mycoparasitism may be different among the plant pathogens. In this study, a secretome analysis was used to identify some extracellular proteins secreted by T. harzianum ALL42 after growth on cell wall of M. phaseolina, Fusarium sp., and R. solani. The secreted proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. A total of 60 T. harzianum ALL42 secreted proteins excised from the gel were analyzed from the three growth conditions. While seven cell wall-induced proteins were identified, more than 53 proteins spots remain unidentified, indicating that these proteins are either novel proteins or proteins that have not yet been sequenced. Endochitinase, β-glucosidase, α-mannosidase, acid phosphatase, α-1,3-glucanase, and proteases were identified in the gel and also detected in the supernatant of culture.

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Acknowledgments

This work was supported by a biotechnology research grant to C.J.U. (CNPq and FUNAPE/UFG) and FINEP/CT-AGRO/Ação Transversal - 01.07.0551.00. V.N.M was supported by CAPES. The authors thank Nuno M.F.M.S. Domingues (UnB/Cel) for her support with MS analysis.

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Authors and Affiliations

  1. Universidade Estadual de Goiás, UnUCET, BR 153, Km 98 Campus Henrique Santillo, Anápolis, GO, CEP 75000-000, Brazil

    Valdirene Neves Monteiro

  2. Universidade Federal de Goiás, Campus Samambaia, ICB II, Cx., Postal 131, Goiânia, GO, CEP 74001-970, Brazil

    Roberto do Nascimento Silva, Andrei Stecca Steindorff & Cirano José Ulhoa

  3. Universidade Católica de Brasília, UCB, Brasília, DF, 70910-900, Brazil

    Fabio Teles Costa

  4. IB-CEL, Universidade de Brasília, Brasília, DF, 70910-900, Brazil

    Eliane Ferreira Noronha, Carlos André Ornelas Ricart & Marcelo Valle de Sousa

  5. Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil

    Marilene Henning Vainstein

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  1. Valdirene Neves Monteiro
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  2. Roberto do Nascimento Silva
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  9. Cirano José Ulhoa
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Correspondence to Cirano José Ulhoa.

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Monteiro, V.N., do Nascimento Silva, R., Steindorff, A.S. et al. New Insights in Trichoderma harzianum Antagonism of Fungal Plant Pathogens by Secreted Protein Analysis. Curr Microbiol 61, 298–305 (2010). https://doi.org/10.1007/s00284-010-9611-8

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  • DOI: https://doi.org/10.1007/s00284-010-9611-8

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