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Lactobacillus and Bifidobacterium Diversity in Horse Feces, Revealed by PCR-DGGE

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Abstract

Lactobacillus equi, Lactobacillus hayakitensis, Lactobacillus johnsonii, and Weissella confusa/cibaria were the dominant species in 12 South African horses. The Bifidobacterium-group was detected in the feces of only one of the 12 horses. Sequencing of the nested-PCR amplicon identified the Bifidobacterium-group as Parascardovia denticolens. Cell numbers of L. equi, L. hayakitensis, and W. confusa/cibaria were consistent in all samples. P. denticolens, Bifidodobacterium pseudolongum, and a phylogenetic relative of Alloscardovia omnicolens were rarely detected. L. equigenerosi, a dominant species in Japanese horses, was detected in the fecal samples of only one horse.

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Acknowledgments

The authors thank Prof. S. Okada (NODAI Culture Collection Center, Tokyo University of Agriculture) for providing the NRIC strains. Dr. A. Endo received a postdoctoral grant from Claude Leon Foundation, Cape Town, South Africa.

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  1. Department of Microbiology, University of Stellenbosch, 7600, Stellenbosch, South Africa

    Akihito Endo, Y. Futagawa-Endo & L. M. T. Dicks

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  1. Akihito Endo
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  2. Y. Futagawa-Endo
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  3. L. M. T. Dicks
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Correspondence to Akihito Endo.

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Endo, A., Futagawa-Endo, Y. & Dicks, L.M.T. Lactobacillus and Bifidobacterium Diversity in Horse Feces, Revealed by PCR-DGGE. Curr Microbiol 59, 651–655 (2009). https://doi.org/10.1007/s00284-009-9498-4

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  • DOI: https://doi.org/10.1007/s00284-009-9498-4

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