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Antimicrobial Susceptibility Testing of Helicobacter pylori Determined by Microdilution Method Using a New Medium

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Abstract

Antibiotic susceptibility testing of Helicobacter pylori isolates was performed by broth microdilution method with MegaCellTM RPMI-1640 Medium (SIGMA). Fifty five clinical isolates of H. pylori were tested against metronidazole, tinidazole, amoxicillin, and clarithromycin. The results were compared to those obtained by standard agar dilution method. The microdilution method performed with new medium, showed excellent correlation with agar dilution results, with 100% agreement for metronidazole, 96.3% for amoxicillin, 90.7% for clarithromycin, and 92.8% for tinidazole. MICs determined by proposed method were highly reproducible: replicate results were variable within one-two-fold dilution by using different inocula and different batches of medium.

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Acknowledgment

This work was supported by financial grant from University (Fondo Interno Ricerca Scientifica E Tecnologica ex 60%).

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  1. Università degli Studi, Sanità Pubblica- Microbiologia-Virologia, Milan, Italy

    Francesca Sisto, Maria Maddalena Scaltrito, Giuseppe Russello, Arianna Bonomi & Francesco Dubini

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  1. Francesca Sisto
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  2. Maria Maddalena Scaltrito
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  3. Giuseppe Russello
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  4. Arianna Bonomi
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  5. Francesco Dubini
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Correspondence to Francesca Sisto.

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Sisto, F., Scaltrito, M.M., Russello, G. et al. Antimicrobial Susceptibility Testing of Helicobacter pylori Determined by Microdilution Method Using a New Medium. Curr Microbiol 58, 559–563 (2009). https://doi.org/10.1007/s00284-009-9368-0

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  • DOI: https://doi.org/10.1007/s00284-009-9368-0

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