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l-Methioninase Production by Filamentous Fungi: I-Screening and Optimization Under Submerged Conditions

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Abstract

Findings show 21 fungal isolates belonging to eight genera recovered from Egyptian soils that have the potential to attack l-methionine under submerged conditions. Aspergillus flavipes had the most methioninolytic activity, giving the highest yield of l-methioninase (10.78 U/mg protein), rate of methionine uptake (93.0%), and growth rate (5.0 g/l), followed by Scopulariopsis brevicaulis and A. carneus. The maximum l-methioninase productivity (11.60 U/mg protein) by A. flavipes was observed using l-methionine (0.8%) as an enzyme-inductive agent and glucose (1%) as a co-dissimilated carbon source. A significant reduction in l-methioninase biosynthesis by A. flavipes was detected using carbon-free medium, suggesting the lack of ability to use l-methionine as a carbon and nitrogen source. Potassium dihydrogen phosphate (0.25%), the best source of phosphorus, favors enzyme biosynthesis and enhances the level of methionine uptake by A. flavipes. The maximum l-methioninase productivity (12.58 U/mg protein) and substrate uptake (95.6%) were measured at an initial pH of 7.0.

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Author notes

  1. Ashraf S. A. El-Sayed

    Present address: Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Sakyo-ku, Kyoto, 606-8585, Japan

Authors and Affiliations

  1. Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt

    Salwa A. Khalaf & Ashraf S. A. El-Sayed

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  1. Salwa A. Khalaf
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  2. Ashraf S. A. El-Sayed
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Correspondence to Ashraf S. A. El-Sayed .

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Khalaf, S.A., El-Sayed , A.S.A. l-Methioninase Production by Filamentous Fungi: I-Screening and Optimization Under Submerged Conditions. Curr Microbiol 58, 219–226 (2009). https://doi.org/10.1007/s00284-008-9311-9

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  • DOI: https://doi.org/10.1007/s00284-008-9311-9

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