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Ethanol production by a filamentous fungal strain Byssochlamys fulva AM130 under alternating aerobic and oxygen-limited conditions

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Abstract

Byssochlamys fulva AM130, a novel strain of filamentous fungus, could produce ethanol from glucose, xylose, and alkali pretreated rice straw (PRS), while the efficiencies were very low with PRS. Ethanol production of 11.84 g/L was attained by the fungus when grown in glucose, indicating that the limitations while growing on PRS were related to low hydrolytic efficiency. Enzyme profiling of the fungus showed 365 IU/ml of beta-glucosidase and 89 IU/ml of xylanase activity, while endoglucanase and filter paper activity were negligible, which accounts for the low hydrolytic efficiency. The fungus could survive for extended periods under oxygen-limited conditions and produce ethanol. The fungal mycelia could also be used for repeated cycles of anaerobic fermentation, wherein the ethanol yield improved with each consecutive cycle.

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Acknowledgements

The authors are thankful to the Council of Scientific and Industrial Research (CSIR) for the financial support for the project MLP 0035 (33/2018/MD-FTT&FTC-ANB) of which this study forms a part of. PKV would like to thank CSIR for providing research fellowship for his Ph.D. work. The authors declare that they do not have any conflict of interest.

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Authors and Affiliations

  1. Biofuels and Biorefineries Section, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram, 695019, India

    Jayaram Krishnamoorthy, Prajeesh Kooloth-Valappil, Velayudhanpillai Prasannakumari Adarsh, Anoop Puthiyamadam & Rajeev K. Sukumaran

  2. Department of Botany, St Peter’s College, Kolencherry, Kerala, India

    Abraham Mathew

  3. Academy of Scientific and Innovative Research (AcSIR), CSIR-National Institute for Interdisciplinary Science and Technology Campus, Thiruvananthapuram, India

    Prajeesh Kooloth-Valappil & Rajeev K. Sukumaran

  4. Centre for Innovation and Translational Research, CSIR-Indian Institute of Toxicology Research, Lucknow, India

    Ashok Pandey

  5. Stelis Biopharma, Doddballapura, Bengaluru, India

    Jayaram Krishnamoorthy

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  1. Jayaram Krishnamoorthy
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  2. Abraham Mathew
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  3. Prajeesh Kooloth-Valappil
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  7. Rajeev K. Sukumaran
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Correspondence to Rajeev K. Sukumaran.

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Krishnamoorthy, J., Mathew, A., Kooloth-Valappil, P. et al. Ethanol production by a filamentous fungal strain Byssochlamys fulva AM130 under alternating aerobic and oxygen-limited conditions. Syst Microbiol and Biomanuf 1, 111–121 (2021). https://doi.org/10.1007/s43393-020-00010-y

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  • DOI: https://doi.org/10.1007/s43393-020-00010-y

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