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Biostraticola tofi gen. nov., spec. nov., A Novel Member of the Family Enterobacteriaceae

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Abstract

Bacterial strain BF36T, isolated from the biofilm of a tufa deposit in a hard water rivulet, was characterized by a polyphasic taxonomic approach. Cells of these organisms were Gram-negative, motile, nonpigmented, rod-shaped, non-endospore-forming, and facultatively anaerobic. Cells, organized in loose consortia, were coated by a massive slime layer. Phylogenetic analyses using 16S rRNA gene sequences showed that strain BF36T was a member of the family Enterobacteriaceae, class Gammaproteobacteria, displaying a moderate degree of relationship (96.5% sequence similarity) to Sodalis glossinidius and “Sodalis pallipedes,” intracellular symbionts of the tsetse fly Glossinis morsitans morsitans. Dendrograms of relationship generated by different algorithms consistently grouped isolate BF36T with Sodalis glossinidius, Pragia fontium, Budvicia aquatica, Serratia rubideae, and Brenneria spp (94.7–95.8% similarity) which also share many common metabolic properties. Differences between strain BF36T and Sodalis glossinidius DSM 13495T are seen in motility and in the pattern of substrates utilized. Membership to the family was also confirmed by a fatty acid profile consisting of major amounts of C16:0 and C16:1ω7, by the presence of isoprenoids of the ubiquinone Q8 and menaquinone MK8 types and a DNA G + C content of 54.2 mol%. The decision to classify strain BF36T into a new genus Biostraticola gen. nov. is based on its distant phylogenetic position as compared to any other representative of the family and the significant phenotypic differences to its nearest phylogenetic neighbor, Sodalis glossinidius. BF36T represents the type species, for which the name Biostraticola tofi sp. nov. is proposed. The type strain is BF36T (DSM 19580T; CIP109699T).

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Acknowledgments

The study was supported by a grant from the German Research Council FOR 571. We thank Hans Trüper for his advice in nomenclatural matters, Brian Tindall for the determination of isoprenoids quinines, and Peter Schumann for the determination of the G + C content of the DNA. The technical skills of Jolantha Swiderski and Iljana Schroeder are highly appreciated. This is publication #24 of the project DFG FOR 571.

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Authors and Affiliations

  1. DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstr. 7B, 38124, Braunschweig, Germany

    Susanne Verbarg, Anja Frühling, Sylvie Cousin, Evelyne Brambilla, Sabine Gronow & Erko Stackebrandt

  2. Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany

    Heinrich Lünsdorf

Authors
  1. Susanne Verbarg
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  2. Anja Frühling
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  3. Sylvie Cousin
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  4. Evelyne Brambilla
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  5. Sabine Gronow
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  6. Heinrich Lünsdorf
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  7. Erko Stackebrandt
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Corresponding author

Correspondence to Erko Stackebrandt.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain BF36T is AM774412.

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Verbarg, S., Frühling, A., Cousin, S. et al. Biostraticola tofi gen. nov., spec. nov., A Novel Member of the Family Enterobacteriaceae. Curr Microbiol 56, 603–608 (2008). https://doi.org/10.1007/s00284-008-9133-9

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  • DOI: https://doi.org/10.1007/s00284-008-9133-9

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