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Clonal Variability and Its Relevance in Generation of New Pathotypes in the Spot Blotch Pathogen, Bipolaris sorokiniana

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Abstract

Spot blotch pathogen Bipolaris sorokiniana of wheat was investigated with threefold objectives: to establish a relationship between morphological and pathological variability of isolates, identify clonal genotype(s) acting as a source for the generation of new variability, and to determine the mechanism of generation of such variability in the pathogen. Isolates were collected from the leaves and seeds of field-grown wheat crop at four different sites in eastern Gangetic plains of India. Eighty-six clonal isolates derived from a single isolate (gray with white patches, Group III), which segregated in an equal proportion of parental and nonparental types, were studied. Morphological characters—i.e., colony morphology, growth rate, and sporulation—were studied along with disease-causing ability of the isolate clones. Clonal isolates were grouped into three categories. Microscopic analysis of nuclei was done to determine the causes of such variability. Morphological variability appeared to be related to the pathological variability. The isolate having epidemic potential appeared different than that acting as the reservoir for variability. The cause of such variability could be attributed either to hyphal fusion and heterokaryosis, nuclear migration and occurrence of multinucleate state, or a combination of these factors. Random Amplified Polymorphic DNA (RAPD) assay suggested that the unique fragments for different groups could be utilized as molecular markers to identify the isolates of specific groups.

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Acknowledgments

We thank the Council of Scientific and Industrial Research, New Delhi for financial support and Prof. S.C. Lakhotia, Department of Zoology, Banaras Hindu University, India for providing a facility with fluorescence microscopy to conduct this study.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221 005, India

    Shree P. Pandey, Sandeep Sharma & A. K. Joshi

  2. Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221 005, India

    R. Chand

  3. Indian Agricultural Statistical Research Institute, New Delhi, 110 012, India

    P. Shahi

Authors
  1. Shree P. Pandey
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  2. Sandeep Sharma
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  3. R. Chand
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  4. P. Shahi
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  5. A. K. Joshi
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Correspondence to A. K. Joshi.

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Pandey, S.P., Sharma, S., Chand, R. et al. Clonal Variability and Its Relevance in Generation of New Pathotypes in the Spot Blotch Pathogen, Bipolaris sorokiniana . Curr Microbiol 56, 33–41 (2008). https://doi.org/10.1007/s00284-007-9034-3

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