Abstract
Rhodovulum sulfidophilum produces carotenoids in the spheroidene pathway. Spheroidene monooxygenase, CrtA, catalyzes the conversion of spheroidene to spheroidenone. crtA-deleted mutants of R. sulfidophilum did not produce spheroidenone and demethylspheroidenone. In these mutants, the ratio of demethylspheroidene to spheroidene increased with exposure to light. One mutant exhibiting a spheroidene-predominant phenotype did not grow under anaerobic-light conditions and was devoid of bacteriochlorophyll a, even under semiaerobic-light conditions There was no difference in the growth of the mutants under aerobic-dark conditions. These data suggest that demethylspheroidene is important for photosynthesis in R. sulfidophilum.
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Acknowledgments
We thank Dr. M.T. Madigan for the Rbc. bogoriensis cells. This work was supported by a Grant-in-Aid from the Japan Society for the Promotion of Science to I.M. (13780423).
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Maeda, I., Yamashiro, H., Yoshioka, D. et al. Unusual Accumulation of Demethylspheroidene in Anaerobic-Phototrophic Growth of crtA-Deleted Mutants of Rhodovulum sulfidophilum. Curr Microbiol 51, 193–197 (2005). https://doi.org/10.1007/s00284-005-4560-3
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DOI: https://doi.org/10.1007/s00284-005-4560-3